Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/22825
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: A transgenic Camelina sativa seed oil effectively replaces fish oil as a dietary source of eicosapentaenoic acid in mice
Authors: Tejera, Noemi
Vauzour, David
Betancor, Monica
Sayanova, Olga
Usher, Sarah
Cochard, Marianne
Rigby, Neil
Ruiz-Lopez, Noemi
Menoyo, David
Tocher, Douglas R
Napier, Johnathan A
Minihane, Anne Marie
Contact Email: d.r.tocher@stir.ac.uk
Keywords: n–3 PUFA
EPA
DHA
Camelina oil
fish oil
sustainability
desaturation
Fads
transgenic
TG sn-2
Issue Date: 1-Feb-2016
Publisher: American Society for Nutrition
Citation: Tejera N, Vauzour D, Betancor M, Sayanova O, Usher S, Cochard M, Rigby N, Ruiz-Lopez N, Menoyo D, Tocher DR, Napier JA & Minihane AM (2016) A transgenic Camelina sativa seed oil effectively replaces fish oil as a dietary source of eicosapentaenoic acid in mice, Journal of Nutrition, 146 (2), pp. 227-235.
Abstract: Background: Fish currently supplies only 40% of the eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) required to allow all individuals globally to meet the minimum intake recommendation of 500 mg/d. Therefore, alternative sustainable sources are needed.  Objective: The main objective was to investigate the ability of genetically engineeredCamelina sativa(20% EPA) oil (CO) to enrich tissue EPA and DHA relative to an EPA-rich fish oil (FO) in mammals.  Methods: Six-week-old male C57BL/6J mice were fed for 10 wk either a palm oil–containing control (C) diet or diets supplemented with EPA-CO or FO, with the C, low-EPA CO (COL), high-EPA CO (COH), low-EPA FO (FOL), and high-EPA FO (FOH) diets providing 0, 0.4, 3.4, 0.3, and 2.9 g EPA/kg diet, respectively. Liver, muscle, and brain were collected for fatty acid analysis, and blood glucose and serum lipids were quantified. The expression of selected hepatic genes involved in EPA and DHA biosynthesis and in modulating their cellular impact was determined.  Results: The oils were well tolerated, with significantly greater weight gain in the COH and FOH groups relative to the C group (P< 0.001). Significantly lower (36–38%) blood glucose concentrations were evident in the FOH and COH mice relative to C mice (P< 0.01). Hepatic EPA concentrations were higher in all EPA groups relative to the C group (P< 0.001), with concentrations of 0.0, 0.4, 2.9, 0.2, and 3.6 g/100 g liver total lipids in the C, COL, COH, FOL, and FOH groups, respectively. Comparable dose-independent enrichments of liver DHA were observed in mice fed CO and FO diets (P< 0.001). Relative to the C group, lower fatty acid desaturase 1 (Fads1) expression (P< 0.005) was observed in the COH and FOH groups. Higher fatty acid desaturase 2 (Fads2), peroxisome proliferator–activated receptor α (Ppara), and peroxisome proliferator–activated receptor γ (Pparg) (P< 0.005) expressions were induced by CO. No impact of treatment on liver X receptor α (Lxra) or sterol regulatory element-binding protein 1c (Srebp1c) was evident.  Conclusions: Oil from transgenicCamelinais a bioavailable source of EPA in mice. These data provide support for the future assessment of this oil in a human feeding trial.
Type: Journal Article
URI: http://hdl.handle.net/1893/22825
DOI Link: http://dx.doi.org/10.3945/​jn.115.223941
Rights: This is an open access article distributed under the CC-BY license (http://creativecommons.org/licenses/by/3.0/).
Affiliation: University of East Anglia
University of East Anglia
Aquaculture
Rothamsted Research
Rothamsted Research
University of East Anglia
Institute of Food Research
Technical University of Madrid
Universidad Politécnica de Madrid
Aquaculture
Rothamsted Research
University of East Anglia

Files in This Item:
File Description SizeFormat 
Tejera et al_Journal of Nutrition_2016.pdf874.88 kBAdobe PDFView/Open


This item is protected by original copyright



Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

If you believe that any material held in STORRE infringes copyright, please contact library@stir.ac.uk providing details and we will remove the Work from public display in STORRE and investigate your claim.