Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/22823
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: The pangenome of the Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV)
Authors: de, Brito Anderson Fernandes
Branconi, Carla Torres
Weidmann, Manfred
Dilcher, Meik
Alves, Jaoa Marcelo Pereira
Gruber, Arthur
Zanotto, Paolo M D A
Contact Email: m.w.weidmann@stir.ac.uk
Keywords: baculovirus
evolution
wild isolates
horizontal gene transfer
deep sequencing
genetic diversity
Issue Date: Jan-2016
Publisher: Oxford University Press
Citation: de Brito AF, Branconi CT, Weidmann M, Dilcher M, Alves JMP, Gruber A & Zanotto PMAD (2016) The pangenome of the Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV), Genome Biology and Evolution, 8 (1), pp. 94-108.
Abstract: The alphabaculovirusAnticarsia gemmatalismultiple nucleopolyhedrovirus (AgMNPV) is the world’s most successful viral bioinsecticide. Through the 1980s and 1990s, this virus was extensively used for biological control of populations ofAnticarsia gemmatalis(Velvetbean caterpillar) in soybean crops. During this period, genetic studies identified several variable loci in the AgMNPV; however, most of them were not characterized at the sequence level. In this study we report a full genome comparison among 17 wild-type isolates of AgMNPV. We found the pangenome of this virus to contain at least 167 hypothetical genes, 151 of which are shared by all genomes. The genebro-athat might be involved in host specificity and carrying transporter is absent in some genomes, and new hypothetical genes were observed. Among these genes there is a uniquernf12-likegene, probably implicated in ubiquitination. Events of gene fission and fusion are common, as four genes have been observed as single or split open reading frames. Gains and losses of genomic fragments (from 20 to 900 bp) are observed within tandem repeats, such as in eight direct repeats and four homologous regions. Most AgMNPV genes present low nucleotide diversity, and variable genes are mainly located in a locus known to evolve through homologous recombination. The evolution of AgMNPV is mainly driven by small indels, substitutions, gain and loss of nucleotide stretches or entire coding sequences. These variations may cause relevant phenotypic alterations, which probably affect the infectivity of AgMNPV. This work provides novel information on genomic evolution of the AgMNPV in particular and of baculoviruses in general.
Type: Journal Article
URI: http://hdl.handle.net/1893/22823
DOI Link: http://dx.doi.org/10.1093/gbe/evv231
Rights: © The Author(s) 2015. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
Affiliation: University of Sao Paulo
University of Sao Paulo
Aquaculture
University of Gottingen, Georg-August University
University of Sao Paulo
University of Sao Paulo
University of Sao Paulo

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