Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/22775
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dc.contributor.authorXie, Dizhien_UK
dc.contributor.authorWang, Shuqien_UK
dc.contributor.authorYou, Cuihongen_UK
dc.contributor.authorChen, Fangen_UK
dc.contributor.authorTocher, Douglas Ren_UK
dc.contributor.authorLi, Yuanyouen_UK
dc.date.accessioned2016-01-27T00:05:51Z-
dc.date.available2016-01-27T00:05:51Z-
dc.date.issued2015-10en_UK
dc.identifier.urihttp://hdl.handle.net/1893/22775-
dc.description.abstractThe present study aimed to characterize the influence of salinity on the biosynthesis of long-chain polyunsaturated fatty acid (LC-PUFA) in rabbitfishSiganus canaliculatus. An eight-week feeding trial was performed in rabbitfish juveniles with diets containing fish oil (FO) or a blend of vegetable oils (perilla and Canola oils, VO) at two salinities, 32 and 10ppt. The whole-body fatty acid mass balance (FAMB) method was used to evaluate thein vivoLC-PUFA biosynthetic activities, and the hepatic mRNA levels of Δ4 and Δ6/Δ5 fatty acyl desaturases (Fad) and elongase of very long-chain fatty acids (Elovl5) genes were determined by real-time quantitative PCR. The results showed that theex novoproduction of LC-PUFA in fish receiving the VO diet was significantly higher than fish fed the FO diet at both salinities. Furthermore, LC-PUFA production at 10ppt salinity was significantly higher than that at 32ppt salinity in the VO dietary groups, whereas no effect of salinity was found in the FO dietary groups. Consistent with this, the calculated apparentin vivodesaturation and elongation activities were also higher in VO and low-salinity treatments. In addition, higher levels ofΔ4andΔ6/Δ5 fadsmRNA expression were obtained at low salinity, which was consistent with the calculated enzyme activities. In contrast, the expression ofelovl5was lower than that offads,and the levels were not consistent with the elongase activity. The results suggest that ambient salinity may affect the activity of the LC-PUFA biosynthetic pathway in rabbitfish through regulating fatty acyl desaturase and elongase activities, partly through a transcriptional control mechanism in the case of desaturases.en_UK
dc.language.isoenen_UK
dc.publisherWiley-Blackwellen_UK
dc.relationXie D, Wang S, You C, Chen F, Tocher DR & Li Y (2015) Characteristics of LC-PUFA biosynthesis in marine herbivorous teleost Siganus canaliculatus under different ambient salinities. Aquaculture Nutrition, 21 (5), pp. 541-551. https://doi.org/10.1111/anu.12178en_UK
dc.rightsThe publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.en_UK
dc.rights.urihttp://www.rioxx.net/licenses/under-embargo-all-rights-reserveden_UK
dc.subjectbiosynthesisen_UK
dc.subjectdesaturaseen_UK
dc.subjectelongaseen_UK
dc.subjectfatty acid mass balanceen_UK
dc.subjectLC-PUFAen_UK
dc.subjectrabbitfishen_UK
dc.subjectsalinityen_UK
dc.titleCharacteristics of LC-PUFA biosynthesis in marine herbivorous teleost Siganus canaliculatus under different ambient salinitiesen_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate2999-12-22en_UK
dc.rights.embargoreason[Xie_et_al-2015-Aquaculture_Nutrition.pdf] The publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work.en_UK
dc.identifier.doi10.1111/anu.12178en_UK
dc.citation.jtitleAquaculture Nutritionen_UK
dc.citation.issn1365-2095en_UK
dc.citation.issn1353-5773en_UK
dc.citation.volume21en_UK
dc.citation.issue5en_UK
dc.citation.spage541en_UK
dc.citation.epage551en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusVoR - Version of Recorden_UK
dc.author.emaild.r.tocher@stir.ac.uken_UK
dc.citation.date21/10/2014en_UK
dc.contributor.affiliationShantou Universityen_UK
dc.contributor.affiliationShantou Universityen_UK
dc.contributor.affiliationShantou Universityen_UK
dc.contributor.affiliationShantou Universityen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationShantou Universityen_UK
dc.identifier.isiWOS:000360939200003en_UK
dc.identifier.scopusid2-s2.0-84940976942en_UK
dc.identifier.wtid588392en_UK
dc.contributor.orcid0000-0002-8603-9410en_UK
dc.date.accepted2014-01-21en_UK
dcterms.dateAccepted2014-01-21en_UK
dc.date.filedepositdate2016-01-26en_UK
rioxxterms.apcnot requireden_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionVoRen_UK
local.rioxx.authorXie, Dizhi|en_UK
local.rioxx.authorWang, Shuqi|en_UK
local.rioxx.authorYou, Cuihong|en_UK
local.rioxx.authorChen, Fang|en_UK
local.rioxx.authorTocher, Douglas R|0000-0002-8603-9410en_UK
local.rioxx.authorLi, Yuanyou|en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate2999-12-22en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/under-embargo-all-rights-reserved||en_UK
local.rioxx.filenameXie_et_al-2015-Aquaculture_Nutrition.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source1353-5773en_UK
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