Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/22733
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Antioxidant activity and lipid peroxidation in Artemia nauplii enriched with a DHA-rich oil emulsion and the effect of adding an external antioxidant based on hydroxytyrosol (Forthcoming/Available Online)
Authors: Viciano, Elena
Monroig, Oscar
Barata, Carlos
Peña, Carlos
Navarro, Juan Carlos
Contact Email: oscar.monroig@stir.ac.uk
Keywords: Antioxidant enzymes
Artemia
docosahexaenoic acid
enrichment
hydroxytyrosol
oxidation
Issue Date: 19-Nov-2015
Publisher: Wiley-Blackwell
Citation: Viciano E, Monroig O, Barata C, Peña C & Navarro JC Antioxidant activity and lipid peroxidation in Artemia nauplii enriched with a DHA-rich oil emulsion and the effect of adding an external antioxidant based on hydroxytyrosol (Forthcoming/Available Online), Aquaculture Research.
Abstract: Artemia nauplii catabolise polyunsaturated fatty acids (PUFA); in particular, they retroconvert docosahexaenoic acid (DHA, 22:6n-3), so enrichment is a continuous quest towards increasing PUFA through the use of PUFA-rich enrichment products. However, optimal conditions during enrichment (aeration, illumination and temperatures around 28 ºC) tend to accelerate autoxidation of PUFA, and the formation of potentially toxic oxidation products. Water-soluble antioxidants like the polyphenolic compound hydroxytyrosol (3,4-dihydroxyphenylethanol), a polar molecule found in the water fraction resulting after the milling process of olives, arise as promising compounds to prevent oxidation during Artemia enrichments. We investigated the antioxidant activity and lipid peroxidation in Artemia nauplii during enrichment, and the effect of adding an external antioxidant based on hydroxytyrosol during the enrichment with a PUFA-rich emulsion (M70). For this purpose, the activity of antioxidant enzymes (catalase, superoxide dismutase, glutathione-S-transferase, glutathione peroxidase), as well as lipid peroxidation, were determined in enriched and unenriched Artemia nauplii. To validate antioxidant activity and lipid peroxidation, in a first experiment, nauplii were enriched with microalgae (Tetraselmis suecica), yeast (Saccharomyces cerevisiae) and M70 emulsion. In a second experiment, enrichment with a commercial emulsion (DC Super Selco), M70, and a combination of M70 and hydroxytyrosol (Hytolive, HYT) added as an external antioxidant was performed. The combination of M70 with HYT produced the best results, in terms of activity of antioxidant enzymes. The analysis of the fatty acids from total lipids showed that the addition of hydroxytyrosol preserved the DHA percentage of enriched nauplii.
Type: Journal Article
URI: http://hdl.handle.net/1893/22733
DOI Link: http://dx.doi.org/10.1111/are.12943
Rights: This item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.
Affiliation: Institute of Aquaculture Torre de la Sal
Complex Systems
Spanish National Research Council (CSIC)
Institute of Environmental Assessment and Water Research (Spain)
Institute of Aquaculture Torre de la Sal

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