|Appears in Collections:||Faculty of Health Sciences and Sport Journal Articles|
|Peer Review Status:||Refereed|
|Title:||Myostatin levels in skeletal muscle of hibernating ground squirrels|
Myburgh, Kathryn H
Storey, Kenneth B
|Citation:||Brooks N, Myburgh KH & Storey KB (2011) Myostatin levels in skeletal muscle of hibernating ground squirrels, Journal of Experimental Biology, 214 (15), pp. 2522-2527.|
|Abstract:||Myostatin, a negative regulator of muscle mass, is elevated during disuse and starvation. Mammalian hibernation presents a unique scenario, where animals are hypocaloric and in torpor, but the extent of muscle protein loss is minimized. We hypothesized that myostatin expression, which is usually increased early in disuse and under hypocaloric conditions, could be suppressed in this unique model. Skeletal muscle was collected from thirteen-lined ground squirrels, Spermophilus tridecemlineatus, at six time points during hibernation: control euthermic (CON); entrance into hibernation (ENT), body temperature (Tb) falling; early hibernation (EHib), stable Tb in torpor for 24 h; late hibernation (LHib), stable Tb in torpor for 3 days; early arousal (EAr), Tb rising; and arousal (AR), Tb restored to 34-37°C for about 18 h. There was no significant increase of myostatin during ENT, EHib or LHib. Unexpectedly, there were approximately sixfold increases in myostatin protein levels as squirrels arose from torpor. The elevation during EAr remained high in AR, which represented an interbout time period. Mechanisms that could release the suppression or promote increased levels of myostatin were assessed. SMAD2 and phosphorylated SMAD2 were increased during EHib, but only the phosphorylated SMAD2 during AR mirrored increases in myostatin. Follistatin, a negative regulator of myostatin, did not follow the same time course as myostatin or its signaling pathway, indicating more control of myostatin at the signaling level. However, SMAD7, an inhibitory SMAD, did not appear to play a significant role during deep hibernation. Hibernation is an excellent natural model to study factors involved in the endogenous intracellular mechanisms controlling myostatin.|
|Rights:||Publisher policy allows this work to be made available in this repository. Published in Journal of Experimental Biology 2011 214: 2522-2527; doi: 10.1242/jeb.055764 by The Company of Biologists. The original publication is available at: http://jeb.biologists.org/content/214/15/2522.short|
|Brooks et al. J Exp Biol. 2011.pdf||486.38 kB||Adobe PDF||View/Open|
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