Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/22290
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Genetic and biological characterization of selected Changuinola viruses (Reoviridae, Orbivirus) from Brazil
Authors: Silva, Sandro P
Dilcher, Meik
Weber, Franziska
Hufert, Frank T
Weidmann, Manfred
Cardoso, Jedson F
Carvalho, Valeria L
Chang, Jannifer O
Martins, Livia C
Lima, Clayton P S
Silva, Daisy E A
Vianez-Junior, Joao L S G
Popov, Vsevolod L
Travassos, da Rosa Amelia P A
Tesh, Robert B
Vasconcelos, Pedro F C
Nunes, Marcio R T
Contact Email: m.w.weidmann@stir.ac.uk
Keywords: Arboviruses
Changuinola virus
Orbivirus
reoviridae
Issue Date: Oct-2014
Publisher: Society for General Microbiology
Citation: Silva SP, Dilcher M, Weber F, Hufert FT, Weidmann M, Cardoso JF, Carvalho VL, Chang JO, Martins LC, Lima CPS, Silva DEA, Vianez-Junior JLGS, Popov VL, Travassos da Rosa APA, Tesh RB, Vasconcelos PFC & Nunes MRT (2014) Genetic and biological characterization of selected Changuinola viruses (Reoviridae, Orbivirus) from Brazil, Journal of General Virology, 95 (10), pp. 2251-2259.
Abstract: The Orbivirus genus of the family Reoviridae is comprised of 22 virus species including the Changuinola virus (CGLV) serogroup. The complete genome sequences of 13 CGLV serotypes isolated between 1961 and 1988 from distinct geographic areas of the Brazilian Amazon region were obtained. All viral sequences were obtained from single-passaged CGLV strains grown in Vero cells. CGLVs are the only orbiviruses known to be transmitted by phlebotomine sandflies. Ultrastructure and molecular analyses by electron microscopy and gel electrophoresis, respectively revealed viral particles with typical orbivirus size and morphology, as well as the presence of a segmented genome with 10 segments. Full-length nucleotide sequencing of each of the ten RNA segments of the 13 CGLV serotypes provided basic information regarding the genome organization, encoded proteins and genetic traits. Segment 2 (encoding VP2) of the CGLV is uncommonly larger in comparison to those found in other orbiviruses and shows varying sizes even among different CGLV serotypes. Phylogenetic analyses support previous serologic findings, which indicated that CGLV constitutes a separate serogroup within the genus Orbivirus. In addition, 6 out of 13 analyzed CGLV serotypes show reassortment of their genome segments.
Type: Journal Article
URI: http://hdl.handle.net/1893/22290
DOI Link: http://dx.doi.org/10.1099/vir.0.064691-0
Rights: The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.
Affiliation: Evandro Chagas Institute
University of Gottingen, Georg-August University
University of Gottingen, Georg-August University
University of Gottingen, Georg-August University
Aquaculture
Evandro Chagas Institute
Evandro Chagas Institute
Evandro Chagas Institute
Evandro Chagas Institute
Evandro Chagas Institute
Evandro Chagas Institute
Evandro Chagas Institute
University of Texas Medical Branch
University of Texas Medical Branch
University of Texas Medical Branch
Evandro Chagas Institute
Evandro Chagas Institute

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