Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/22283
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Molecular mechanism of dietary phospholipid requirement of Atlantic salmon, Salmo salar, fry
Authors: Carmona-Antonanzas, Greta
Taylor, John
Martinez, Rubio Laura
Tocher, Douglas R
Contact Email: drt1@stir.ac.uk
Keywords: Atlantic salmon
dietary requirement
gene expression
phosphoglycerides
phospholipids
qPCR
Issue Date: Nov-2015
Publisher: Elsevier
Citation: Carmona-Antonanzas G, Taylor J, Martinez Rubio L & Tocher DR (2015) Molecular mechanism of dietary phospholipid requirement of Atlantic salmon, Salmo salar, fry, Biochimica et Biophysica Acta (BBA)- Molecular and Cell Biology of Lipids, 1851 (11), pp. 1428-1441.
Abstract: The phospholipid (PL) requirement in fish is revealed by enhanced performance when larvae are provided PL-enriched diets. To elucidate the molecular mechanism underlying PL requirement in Atlantic salmon, Salmo salar, were fed a minimal PL diet and tissue samples from major lipid metabolic sites were dissected from fry and parr. In silico analysis and cloning techniques demonstrated that salmon possess a full set of enzymes for the endogenous production of PL. The gene expression data indicated that major PL biosynthetic genes of phosphatidylcholine (PtdCho), phosphatidylethanolamine (PtdEtn) and phosphatidylinositol (PtdIns) display lower expression in intestine during the early developmental stage (fry). This is consistent with the hypothesis that the intestine of salmon is immature at the early developmental stage with limited capacity for endogenous PL biosynthesis. The results also indicate that intact PtdCho, PtdEtn and PtdIns are required in the diet at this stage. PtdCho and sphimgomyelin constitute the predominant PL in chylomicrons, involved in the transport of dietary lipids from the intestine to the rest of the body. As sphingomyelin can be produced from PtdCho in intestine of fry, our findings suggest that supplementation of dietary PtdCho alone during early developmental stages of Atlantic salmon would be sufficient to promote chylomicron formation. This would support efficient transport of dietary lipids, including PL precursors, from the intestine to the liver where biosynthesis of PtdEtn, PtdSer, and PtdIns is not compromised as in intestine facilitating efficient utilisation of dietary energy and the endogenous production of membrane PL for the rapidly growing and developing animal.
Type: Journal Article
URI: http://hdl.handle.net/1893/22283
DOI Link: http://dx.doi.org/10.1016/j.bbalip.2015.08.006
Rights: This item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Accepted refereed manuscript of: Carmona-Antoñanzas G, Taylor J, Martinez Rubio L & Tocher DR (2015) Molecular mechanism of dietary phospholipid requirement of Atlantic salmon, Salmo salar, fry, Biochimica et Biophysica Acta (BBA)- Molecular and Cell Biology of Lipids, 1851 (11), pp. 1428-1441. DOI: 10.1016/j.bbalip.2015.08.006 © 2015, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/
Affiliation: Aquaculture
Aquaculture
Aquaculture
Aquaculture

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