Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/22056
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dc.contributor.authorMonroig, Oscaren_UK
dc.contributor.authorHontoria, Franciscoen_UK
dc.contributor.authorVaro, Inmaen_UK
dc.contributor.authorTocher, Douglas Ren_UK
dc.contributor.authorNavarro, Juan Carlosen_UK
dc.date.accessioned2015-12-18T00:28:37Z-
dc.date.available2015-12-18T00:28:37Z-
dc.date.issued2016-01-01en_UK
dc.identifier.urihttp://hdl.handle.net/1893/22056-
dc.description.abstractSome polyunsaturated fatty acids (PUFA) play critical roles in physiology and are essential for normal growth and development of aquatic animals including cephalopods like the common cuttlefish Sepia officinalis. This is particularly true for early life stages when neuronal tissues are rapidly developing and accumulating PUFA. The specific fatty acids (FAs) that can satisfy the essential requirements of any animal species depend upon the enzymatic capability to convert dietary FA into those physiologically important FAs required for normal function. In order to identify the dietary essential FA for the common cuttlefish, we have performed the molecular cloning and functional characterisation of two key enzymes involved in PUFA biosynthesis in this species, namely a fatty acyl desaturase (Fad) and an elongation of very long-chain fatty acid (Elovl) protein. The gene product of the cuttlefish Fad exhibited ∆5-desaturase activity, enabling this species to potentially biosynthesise the physiologically essential FAs eicosapentaenoic (20:5n-3, EPA) and arachidonic (20:4n-3, ARA) acids from 20:4n-3 and 20:3n-6, respectively. However, the cuttlefish Fad did not show ∆6 or ∆8 activity and therefore the biosynthesis of EPA and ARA from C18 PUFA precursors could be limited, suggesting that both may be essential FAs for cuttlefish. Moreover, the cuttlefish Fad also lacked ∆4-desaturase activity suggesting that DHA biosynthesis was not possible and thus DHA is also an essential FA for this species. The cuttlefish ∆5 Fad was able to produce non-methylene-interrupted (NMI) FA, a group of PUFAs typically found in marine invertebrates. The cuttlefish Elovl was able to elongate C18 and C20 PUFA substrates, but showed no activity towards C22 PUFA. Overall the results obtained in the present study allowed the prediction of the biosynthetic pathways of PUFA, including NMI FA, in the common cuttlefish. It was concluded that EPA, ARA and DHA were likely to be essential dietary FA for this species as endogenous production from precursor FA appears to be limited.en_UK
dc.language.isoenen_UK
dc.publisherElsevieren_UK
dc.relationMonroig O, Hontoria F, Varo I, Tocher DR & Navarro JC (2016) Investigating the essential fatty acids in the common cuttlefish Sepia officinalis (Mollusca, Cephalopoda): Molecular cloning and functional characterisation of fatty acyl desaturase and elongase. Aquaculture, 450, pp. 38-47. https://doi.org/10.1016/j.aquaculture.2015.07.003en_UK
dc.rightsThis item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Accepted refereed manuscript of: Monroig O, Hontoria F, Varo I, Tocher DR & Navarro JC (2016) Investigating the essential fatty acids in the common cuttlefish Sepia officinalis (Mollusca, Cephalopoda): Molecular cloning and functional characterisation of fatty acyl desaturase and elongase, Aquaculture, 450, pp. 38-47. DOI: 10.1016/j.aquaculture.2015.07.003 © 2015, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/en_UK
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en_UK
dc.subjectElongaseen_UK
dc.subjectessential fatty acidsen_UK
dc.subjectdesaturaseen_UK
dc.subjectpolyunsaturated fatty acidsen_UK
dc.subjectSepia officinalisen_UK
dc.titleInvestigating the essential fatty acids in the common cuttlefish Sepia officinalis (Mollusca, Cephalopoda): Molecular cloning and functional characterisation of fatty acyl desaturase and elongaseen_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate2016-12-09en_UK
dc.rights.embargoreason[Monroig et al 2015 (Sepia).pdf] Publisher requires embargo of 12 months after formal publication.en_UK
dc.identifier.doi10.1016/j.aquaculture.2015.07.003en_UK
dc.citation.jtitleAquacultureen_UK
dc.citation.issn0044-8486en_UK
dc.citation.volume450en_UK
dc.citation.spage38en_UK
dc.citation.epage47en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusAM - Accepted Manuscripten_UK
dc.author.emailoscar.monroig@stir.ac.uken_UK
dc.citation.date08/07/2015en_UK
dc.contributor.affiliationComplex Systems - LEGACYen_UK
dc.contributor.affiliationInstitute of Aquaculture Torre de la Salen_UK
dc.contributor.affiliationInstitute of Aquaculture Torre de la Salen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquaculture Torre de la Salen_UK
dc.identifier.isiWOS:000364854000007en_UK
dc.identifier.scopusid2-s2.0-84937053158en_UK
dc.identifier.wtid594813en_UK
dc.contributor.orcid0000-0001-8712-0440en_UK
dc.contributor.orcid0000-0002-8603-9410en_UK
dc.date.accepted2015-07-07en_UK
dcterms.dateAccepted2015-07-07en_UK
dc.date.filedepositdate2015-07-17en_UK
rioxxterms.apcnot requireden_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionAMen_UK
local.rioxx.authorMonroig, Oscar|0000-0001-8712-0440en_UK
local.rioxx.authorHontoria, Francisco|en_UK
local.rioxx.authorVaro, Inma|en_UK
local.rioxx.authorTocher, Douglas R|0000-0002-8603-9410en_UK
local.rioxx.authorNavarro, Juan Carlos|en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate2016-12-09en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/under-embargo-all-rights-reserved||2016-12-08en_UK
local.rioxx.licencehttp://creativecommons.org/licenses/by-nc-nd/4.0/|2016-12-09|en_UK
local.rioxx.filenameMonroig et al 2015 (Sepia).pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source0044-8486en_UK
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