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Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Molecular and serological findings in suspected patients with Crimean-Congo hemorrhagic fever virus in Iran
Authors: Karlberg, Helen
Sharifi-Mood, Batool
Mousavi-Jazi, Mehrdad
Dilcher, Meik
Lindegren, Gunnel
Mardani, Masoud
Bereskly, Sandor
Weidmann, Manfred
Mirazimi, Ali
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Keywords: CCHF
neutralization assay
Issue Date: Apr-2015
Publisher: Wiley-Blackwell
Citation: Karlberg H, Sharifi-Mood B, Mousavi-Jazi M, Dilcher M, Lindegren G, Mardani M, Bereskly S, Weidmann M & Mirazimi A (2015) Molecular and serological findings in suspected patients with Crimean-Congo hemorrhagic fever virus in Iran, Journal of Medical Virology, 87 (4), pp. 686-693.
Abstract: Crimean-Congo hemorrhagic fever (CCHF) is an arthropod-borne disease of humans associated with a severe clinical picture, including hemorrhagic syndrome and a high mortality rate. CCHF virus is widely distributed throughout large areas of the world. To characterize the serological status in CCHF patients, paired clinical samples were collected from suspected CCHF patients and analyzed by microbiological and other laboratory analyses with the aim of: determining the presence of neutralizing antibodies against CCHF virus; investigating the cross-reactivity of these neutralizing antibodies against virus isolated from the same outbreak and against other available laboratory strain; and studying the relationship between the isolated virus with other virus by whole genome sequencing. Patients at Boo-Ali Hospital, Zahedan, Iran, with clinical symptoms ranging from mild to severe hemorrhagic fever were included in the study. Two serum samples were taken from each patient, the first as soon as the patient matched the criteria for CCHF notification and the second when the patient was discharged from hospital (2 weeks later). Commercial and in-house assays revealed a positive IgM signal in acute serum samples from six patients. A novel finding was that CCHF patients develop neutralizing antibodies soon after infection. Interestingly these antibodies were able to neutralize other CCHF virus strains too. The complete sequence of the Zahedan 2007 isolate, including the hitherto unknown first L-segment sequence, was identified using an original clinical sample from one patient with confirmed CCHF infection.
Type: Journal Article
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Affiliation: Karolinska Institutet
Zahedan University of Medical Sciences
Public Health Agency of Sweden
University of Gottingen, Georg-August University
Public Health Agency of Sweden
Shahid Beheshti University of Medical Sciences
Public Health Agency of Sweden
Swedish Institute for Infectious Disease Control

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