Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/21705
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Rapid detection of infectious hypodermal and hematopoietic necrosis virus (IHHNV) by real-time, isothermal recombinase polymerase amplification assay
Authors: Xia, Xiaoming
Yu, Yongxin
Hu, Linghao
Weidmann, Manfred
Pan, Yingjie
Yan, Shuling
Wang, Yongjie
Contact Email: m.w.weidmann@stir.ac.uk
Issue Date: Apr-2015
Publisher: Springer
Citation: Xia X, Yu Y, Hu L, Weidmann M, Pan Y, Yan S & Wang Y (2015) Rapid detection of infectious hypodermal and hematopoietic necrosis virus (IHHNV) by real-time, isothermal recombinase polymerase amplification assay, Archives of Virology, 160 (4), pp. 987-994.
Abstract: Infectious hypodermal and hematopoietic necrosis virus (IHHNV) causes mortality or runt deformity syndrome in penaeid shrimps and is responsible for significant economic losses in the shrimp aquaculture industry. Here, we describe a novel real-time isothermal recombinase polymerase amplification (RPA) assay developed for IHHNV detection. Using IHHNV plasmid standards and DNA samples from a variety of organisms, we evaluated the ability of the IHHNV-RPA assay to detect IHHNV based on analysis of its sensitivity, specificity, rapidity, and reproducibility. Probit analysis of eight independent experimental replicates indicated satisfactory performance of the RPA assay, which is sufficiently sensitive to detect as few as 4 copies of the IHHNV genome within 7min at 39°C with 95% reliability. Therefore, this rapid RPA method has great potential for applications, either in field use or as a point of care diagnostic technique.
Type: Journal Article
URI: http://hdl.handle.net/1893/21705
DOI Link: http://dx.doi.org/10.1007/s00705-015-2357-7
Rights: The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.
Affiliation: Shanghai Ocean University
Shanghai Ocean University
Shanghai Ocean University
Aquaculture
Shanghai Ocean University
University of Gottingen, Georg-August University
Shanghai Ocean University

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