Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/21694
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dc.contributor.authorShepherd, Sam Oen_UK
dc.contributor.authorCocks, Matthewen_UK
dc.contributor.authorTipton, Kevinen_UK
dc.contributor.authorWitard, Oliveren_UK
dc.contributor.authorRanasinghe, Aaron Men_UK
dc.contributor.authorBarker, Thomas Aen_UK
dc.contributor.authorWagenmakers, Anton J Men_UK
dc.contributor.authorShaw, Christopher Sen_UK
dc.date.accessioned2016-09-15T00:55:03Z-
dc.date.available2016-09-15T00:55:03Z-
dc.date.issued2014-06en_UK
dc.identifier.urihttp://hdl.handle.net/1893/21694-
dc.description.abstractNew Findings What is the central question of this study? Recent research from our laboratory, supported by in vitro effects of perilipins, suggested that improvements in insulin sensitivity following endurance training are mechanistically linked to increases in muscle oxidative capacity, intramuscular triglyceride utilization during moderate endurance exercise and increases in the content of the lipid droplet-associated perilipins 2 and 5. This study aimed to investigate whether these adaptations also occur in response to resistance training. What is the main finding and its importance? Six weeks of resistance training increased all the mentioned variables. These novel data suggest that improvements in muscle oxidative capacity and lipid metabolism contribute to the increase in insulin sensitivity following resistance training. Recent in vitro and in vivo experimental observations suggest that improvements in insulin sensitivity following endurance training are mechanistically linked to increases in muscle oxidative capacity, intramuscular triglyceride (IMTG) utilization during endurance exercise and increases in the content of the lipid droplet-associated perilipin2 (PLIN2) and perilipin5 (PLIN5). This study investigated the hypothesis that similar adaptations may also underlie the resistance training (RT)-induced improvements in insulin sensitivity. Thirteen sedentary men (20±1years old; body mass index 24.8±0.8kgm-2) performed 6weeks of whole-body RT (three times per week), and changes in peak O2 uptake (in millilitres per minute per kilogram) and insulin sensitivity were assessed. Muscle biopsies (n=8) were obtained before and after 60min steady-state cycling at ∼65% peak O2 uptake. Immunofluorescence microscopy was used to assess changes in oxidative capacity (measured as cytochrome c oxidase protein content), IMTG and PLIN2 and PLIN5 protein content. Resistance training increased peak O2 uptake (by 8±3%), COX protein content (by 46±13 and 61±13% in type I and II fibres, respectively) and the Matsuda insulin sensitivity index (by 47±6%; all P<0.05). In typeI fibres, IMTG (by 52±11%; P<0.05) and PLIN2 content (by 107±19%; P<0.05) were increased and PLIN5 content tended to increase (by 54±22%; P=0.054) post-training. In typeII fibres, PLIN2 content increased (by 57±20%; P<0.05) and IMTG (by 46±17%; P=0.1) and PLIN5 content (by 44±24%; P=0.054) tended to increase post-training. Breakdown of IMTG during moderate-intensity exercise was greater in both typeI and typeII fibres (by 43±5 and 37±5%, respectively; P<0.05) post-RT. The results confirm the hypothesis that RT enhances muscle oxidative capacity and increases IMTG breakdown and the content of PLIN2 and PLIN5 in both typeI and typeII fibres during endurance-type exercise.en_UK
dc.language.isoenen_UK
dc.publisherWiley-Blackwellen_UK
dc.relationShepherd SO, Cocks M, Tipton K, Witard O, Ranasinghe AM, Barker TA, Wagenmakers AJM & Shaw CS (2014) Resistance training increases skeletal muscle oxidative capacity and net intramuscular triglyceride breakdown in type I and II fibres of sedentary males. Experimental Physiology, 99 (6), pp. 894-908. https://doi.org/10.1113/expphysiol.2014.078014en_UK
dc.rightsThe publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.en_UK
dc.rights.urihttp://www.rioxx.net/licenses/under-embargo-all-rights-reserveden_UK
dc.titleResistance training increases skeletal muscle oxidative capacity and net intramuscular triglyceride breakdown in type I and II fibres of sedentary malesen_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate2999-12-31en_UK
dc.rights.embargoreason[Shepherd Witard 14 RT trig Exp Phys.pdf] The publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work.en_UK
dc.identifier.doi10.1113/expphysiol.2014.078014en_UK
dc.citation.jtitleExperimental Physiologyen_UK
dc.citation.issn1469-445Xen_UK
dc.citation.issn0958-0670en_UK
dc.citation.volume99en_UK
dc.citation.issue6en_UK
dc.citation.spage894en_UK
dc.citation.epage908en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusVoR - Version of Recorden_UK
dc.author.emailoliver.witard@stir.ac.uken_UK
dc.contributor.affiliationLiverpool John Moores Universityen_UK
dc.contributor.affiliationLiverpool John Moores Universityen_UK
dc.contributor.affiliationSporten_UK
dc.contributor.affiliationSporten_UK
dc.contributor.affiliationUniversity of Birminghamen_UK
dc.contributor.affiliationUniversity of Birminghamen_UK
dc.contributor.affiliationLiverpool John Moores Universityen_UK
dc.contributor.affiliationVictoria Universityen_UK
dc.identifier.isiWOS:000337524500007en_UK
dc.identifier.scopusid2-s2.0-84901503130en_UK
dc.identifier.wtid600227en_UK
dc.contributor.orcid0000-0002-6545-8122en_UK
dc.contributor.orcid0000-0002-5875-8397en_UK
dcterms.dateAccepted2014-06-30en_UK
dc.date.filedepositdate2015-04-24en_UK
rioxxterms.apcnot requireden_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionVoRen_UK
local.rioxx.authorShepherd, Sam O|en_UK
local.rioxx.authorCocks, Matthew|en_UK
local.rioxx.authorTipton, Kevin|0000-0002-6545-8122en_UK
local.rioxx.authorWitard, Oliver|0000-0002-5875-8397en_UK
local.rioxx.authorRanasinghe, Aaron M|en_UK
local.rioxx.authorBarker, Thomas A|en_UK
local.rioxx.authorWagenmakers, Anton J M|en_UK
local.rioxx.authorShaw, Christopher S|en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate2999-12-31en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/under-embargo-all-rights-reserved||en_UK
local.rioxx.filenameShepherd Witard 14 RT trig Exp Phys.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source0958-0670en_UK
Appears in Collections:Faculty of Health Sciences and Sport Journal Articles

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