Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/21421
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Targeting and stimulation of the zebrafish (Danio rerio) innate immune system with LPS/dsRNA-loaded nanoliposomes
Authors: Ruyra, Angels
Cano-Sarabia, Mary
Garcia-Valtanen, Pablo
Yero, Daniel
Gibert, Isidre
MacKenzie, Simon
Estepa, Amparo
Maspoch, Daniel
Roher, Nerea
Contact Email: simon.mackenzie@stir.ac.uk
Keywords: Nanoencapsulation
Liposome
Immunostimulant
Innate immunity
Macrophage
Zebrafish
Infection model
Issue Date: 30-Jun-2014
Publisher: Elsevier
Citation: Ruyra A, Cano-Sarabia M, Garcia-Valtanen P, Yero D, Gibert I, MacKenzie S, Estepa A, Maspoch D & Roher N (2014) Targeting and stimulation of the zebrafish (Danio rerio) innate immune system with LPS/dsRNA-loaded nanoliposomes, Vaccine, 32 (31), pp. 3955-3962.
Abstract: Herein we report the use of immunostimulant-loaded nanoliposomes (called NLcliposomes) as a strategy to protect fish against bacterial and/or viral infections. This work entailed developing a method for in vivo tracking of the liposomes administered to adult zebrafish that enables evaluation of their in vivo dynamics and characterisation of their tissue distribution. The NLc liposomes, which co-encapsulate poly(I:C) and LPS, accumulate in immune tissues and in immunologically relevant cells such as macrophages, as has been assessed in trout primary cell cultures. They protect zebrafish against otherwise lethal bacterial (Pseudomonas aeruginosa PAO1) and viral (Spring Viraemia of Carp Virus) infections regardless of whether they are administered by injection or by immersion, as demonstrated in a series of in vivo infection experiments with adult zebrafish. Importantly, protection was not achieved in fish that had been treated with empty liposomes or with a mixture of the free immunostimulants. Our findings indicate that stimulation of the innate immune system with co-encapsulated immunostimulants in nano-liposomes is a promising strategy to simultaneously improve the levels of protection against bacterial and viral infections in fish.
Type: Journal Article
URI: http://hdl.handle.net/1893/21421
DOI Link: http://dx.doi.org/10.1016/j.vaccine.2014.05.010
Rights: © 2014 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).
Affiliation: Universitat Autonoma de Barcelona
ICN2 (Institut Catala de Nanociencia i Nanotecnologia)
The Miguel Hernandez University of Elche
Universitat Autonoma de Barcelona
Universitat Autonoma de Barcelona
Complex Systems
The Miguel Hernandez University of Elche
ICN2 (Institut Catala de Nanociencia i Nanotecnologia)
Universitat Autonoma de Barcelona

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