Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/21416
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Comparative analysis of the acute response of the trout, O. mykiss, head kidney to in vivo challenge with virulent and attenuated infectious hematopoietic necrosis virus and LPS-induced inflammation
Authors: MacKenzie, Simon
Balasch, Joan C
Novoa, Beatriz
Ribas, Laia
Roher, Nerea
Krasnov, Aleksei
Figueras, Antonio
Contact Email: simon.mackenzie@stir.ac.uk
Issue Date: 26-Mar-2008
Publisher: BioMed Central
Citation: MacKenzie S, Balasch JC, Novoa B, Ribas L, Roher N, Krasnov A & Figueras A (2008) Comparative analysis of the acute response of the trout, O. mykiss, head kidney to in vivo challenge with virulent and attenuated infectious hematopoietic necrosis virus and LPS-induced inflammation, BMC Genomics, 9, Art. No.: 141.
Abstract: Background: The response of the trout, O. mykiss, head kidney to bacterial lipopolysaccharide (LPS) or active and attenuated infectious hematopoietic necrosis virus (IHNV and attINHV respectively) intraperitoneal challenge, 24 and 72 hours post-injection, was investigated using a salmonid-specific cDNA microarray. Results: The head kidney response to i.p. LPS-induced inflammation in the first instance displays an initial stress reaction involving suppression of major cellular processes, including immune function, followed by a proliferative hematopoietic-type/biogenesis response 3 days after administration. The viral response at the early stage of infection highlights a suppression of hematopoietic and protein biosynthetic function and a stimulation of immune response. In fish infected with IHNV a loss of cellular function including signal transduction, cell cycle and transcriptional activity 72 hours after infection reflects the tissue-specific pathology of IHNV infection. attIHNV treatment on the other hand shows a similar pattern to native IHNV infection at 24 hours however at 72 hours a divergence from the viral response is seen and replace with a recovery response more similar to that observed for LPS is observed. Conclusion: In conclusion we have been able to identify and characterise by transcriptomic analysis two different types of responses to two distinct immune agents, a virus, IHNV and a bacterial cell wall component, LPS and a 'mixed' response to an attenuated IHNV. This type of analysis will lead to a greater understanding of the physiological response and the development of effective immune responses in salmonid fish to different pathogenic and pro-inflammatory agents.
Type: Journal Article
URI: http://hdl.handle.net/1893/21416
DOI Link: http://dx.doi.org/10.1186/1471-2164-9-141
Rights: © 2008 MacKenzie et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Affiliation: Complex Systems
Universitat Autonoma de Barcelona
Spanish National Research Council (CSIC)
Imperial College London
Universitat de Barcelona
Akvaforsk Genetics Center AS
Spanish National Research Council (CSIC)

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