Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/21410
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: A Novel Liposome-Based Nanocarrier Loaded with an LPS-dsRNA Cocktail for Fish Innate Immune System Stimulation
Authors: Ruyra, Angels
Cano-Sarabia, Mary
MacKenzie, Simon
Maspoch, Daniel
Roher, Nerea
Contact Email: simon.mackenzie@stir.ac.uk
Issue Date: 18-Oct-2013
Publisher: Public Library of Science
Citation: Ruyra A, Cano-Sarabia M, MacKenzie S, Maspoch D & Roher N (2013) A Novel Liposome-Based Nanocarrier Loaded with an LPS-dsRNA Cocktail for Fish Innate Immune System Stimulation, PLoS ONE, 8 (10), Art. No.: e76338.
Abstract: Development of novel systems of vaccine delivery is a growing demand of the aquaculture industry. Nano- and micro- encapsulation systems are promising tools to achieve efficient vaccines against orphan vaccine fish diseases. In this context, the use of liposomal based-nanocarriers has been poorly explored in fish; although liposomal nanocarriers have successfully been used in other species. Here, we report a new ~125 nm-in-diameter unilamellar liposome-encapsulated immunostimulant cocktail containing crude lipopolysaccharide (LPS) from E. coli and polyinosinic:polycytidylic acid [poly (I:C)], a synthetic analog of dsRNA virus, aiming to be used as a non-specific vaccine nanocarrier in different fish species. This liposomal carrier showed high encapsulation efficiencies and low toxicity not only in vitro using three different cellular models but also in vivo using zebrafish embryos and larvae. We showed that such liposomal LPS-dsRNA cocktail is able to enter into contact with zebrafish hepatocytes (ZFL cell line) and trout macrophage plasma membranes, being preferentially internalized through caveolae-dependent endocytosis, although clathrin-mediated endocytosis in ZFL cells and macropinocytocis in macrophages also contribute to liposome uptake. Importantly, we also demonstrated that this liposomal LPS-dsRNA cocktail elicits a specific pro-inflammatory and anti-viral response in both zebrafish hepatocytes and trout macrophages. The design of a unique delivery system with the ability to stimulate two potent innate immunity pathways virtually present in all fish species represents a completely new approach in fish health.
Type: Journal Article
URI: http://hdl.handle.net/1893/21410
DOI Link: http://dx.doi.org/10.1371/journal.pone.0076338
Rights: © 2013 Ruyra et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Affiliation: Universitat Autonoma de Barcelona
ICN2 (Institut Catala de Nanociencia i Nanotecnologia)
Complex Systems
ICN2 (Institut Catala de Nanociencia i Nanotecnologia)
Universitat Autonoma de Barcelona

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