Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/21408
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dc.contributor.authorFerrer-Navarro, Marioen_UK
dc.contributor.authorPlanell, Raquelen_UK
dc.contributor.authorYero, Danielen_UK
dc.contributor.authorMongiardini, Eliasen_UK
dc.contributor.authorTorrent, Gerarden_UK
dc.contributor.authorHuedo, Polen_UK
dc.contributor.authorMartinez, Paulaen_UK
dc.contributor.authorRoher, Nereaen_UK
dc.contributor.authorMacKenzie, Simonen_UK
dc.contributor.authorGibert, Isidreen_UK
dc.contributor.authorDaura, Xavieren_UK
dc.date.accessioned2015-01-28T23:11:01Z-
dc.date.available2015-01-28T23:11:01Z-
dc.date.issued2013-06-26en_UK
dc.identifier.othere67207en_UK
dc.identifier.urihttp://hdl.handle.net/1893/21408-
dc.description.abstractStenotrophomonas maltophilia is a Gram-negative pathogen with emerging nosocomial incidence. Little is known about its pathogenesis and the genomic diversity exhibited by clinical isolates complicates the study of pathogenicity and virulence factors. Here, we present a strategy to identify such factors in new clinical isolates of S. maltophilia, incorporating an adult-zebrafish model of S. maltophilia infection to evaluate relative virulence coupled to 2D difference gel electrophoresis to explore underlying differences in protein expression. In this study we report upon three recent clinical isolates and use the collection strain ATCC13637 as a reference. The adult-zebrafish model shows discrimination capacity, i.e. from very low to very high mortality rates, with clinical symptoms very similar to those observed in natural S. maltophilia infections in fish. Strain virulence correlates with resistance to human serum, in agreement with previous studies in mouse and rat and therefore supporting zebrafish as a replacement model. Despite its clinical origin, the collection strain ATCC13637 showed obvious signs of attenuation in zebrafish, with null mortality. Multilocus-sequence-typing analysis revealed that the most virulent strains, UV74 and M30, exhibit the strongest genetic similitude. Differential proteomic analysis led to the identification of 38 proteins with significantly different abundance in the three clinical strains relative to the reference strain. Orthologs of several of these proteins have been already reported to have a role in pathogenesis, virulence or resistance mechanisms thus supporting our strategy. Proof of concept is further provided by protein Ax21, whose abundance is shown here to be directly proportional to mortality in the zebrafish infection model. Indeed, recent studies have demonstrated that this protein is a quorum-sensing-related virulence factor.en_UK
dc.language.isoenen_UK
dc.publisherPublic Library of Scienceen_UK
dc.relationFerrer-Navarro M, Planell R, Yero D, Mongiardini E, Torrent G, Huedo P, Martinez P, Roher N, MacKenzie S, Gibert I & Daura X (2013) Abundance of the Quorum-Sensing Factor Ax21 in Four Strains of Stenotrophomonas maltophilia Correlates with Mortality Rate in a New Zebrafish Model of Infection. PLoS ONE, 8 (6), Art. No.: e67207. https://doi.org/10.1371/journal.pone.0067207en_UK
dc.rights© 2013 Ferrer-Navarro et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_UK
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_UK
dc.titleAbundance of the Quorum-Sensing Factor Ax21 in Four Strains of Stenotrophomonas maltophilia Correlates with Mortality Rate in a New Zebrafish Model of Infectionen_UK
dc.typeJournal Articleen_UK
dc.identifier.doi10.1371/journal.pone.0067207en_UK
dc.citation.jtitlePLoS ONEen_UK
dc.citation.issn1932-6203en_UK
dc.citation.volume8en_UK
dc.citation.issue6en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusVoR - Version of Recorden_UK
dc.author.emailsimon.mackenzie@stir.ac.uken_UK
dc.contributor.affiliationUniversitat Autonoma de Barcelonaen_UK
dc.contributor.affiliationUniversitat Autonoma de Barcelonaen_UK
dc.contributor.affiliationUniversitat Autonoma de Barcelonaen_UK
dc.contributor.affiliationUniversitat Autonoma de Barcelonaen_UK
dc.contributor.affiliationUniversitat Autonoma de Barcelonaen_UK
dc.contributor.affiliationUniversitat Autonoma de Barcelonaen_UK
dc.contributor.affiliationUniversitat Autonoma de Barcelonaen_UK
dc.contributor.affiliationUniversitat Autonoma de Barcelonaen_UK
dc.contributor.affiliationComplex Systems - LEGACYen_UK
dc.contributor.affiliationUniversitat Autonoma de Barcelonaen_UK
dc.contributor.affiliationUniversitat Autonoma de Barcelonaen_UK
dc.identifier.isiWOS:000321424400085en_UK
dc.identifier.scopusid2-s2.0-84879486284en_UK
dc.identifier.wtid605913en_UK
dc.contributor.orcid0000-0003-1845-6826en_UK
dc.date.accepted2013-05-15en_UK
dcterms.dateAccepted2013-05-15en_UK
dc.date.filedepositdate2015-01-28en_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionVoRen_UK
local.rioxx.authorFerrer-Navarro, Mario|en_UK
local.rioxx.authorPlanell, Raquel|en_UK
local.rioxx.authorYero, Daniel|en_UK
local.rioxx.authorMongiardini, Elias|en_UK
local.rioxx.authorTorrent, Gerard|en_UK
local.rioxx.authorHuedo, Pol|en_UK
local.rioxx.authorMartinez, Paula|en_UK
local.rioxx.authorRoher, Nerea|en_UK
local.rioxx.authorMacKenzie, Simon|0000-0003-1845-6826en_UK
local.rioxx.authorGibert, Isidre|en_UK
local.rioxx.authorDaura, Xavier|en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate2015-01-28en_UK
local.rioxx.licencehttp://creativecommons.org/licenses/by/4.0/|2015-01-28|en_UK
local.rioxx.filenameMacKenzie_PlosOne 2013.pdfen_UK
local.rioxx.filecount1en_UK
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