Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/21212
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dc.contributor.authorDezfuli, Bahram Sen_UK
dc.contributor.authorGiari, Luisaen_UK
dc.contributor.authorLui, Aliceen_UK
dc.contributor.authorSquerzanti, Samanthaen_UK
dc.contributor.authorCastaldelli, Giuseppeen_UK
dc.contributor.authorShinn, Andrewen_UK
dc.contributor.authorManera, Maurizioen_UK
dc.contributor.authorLorenzoni, Massimoen_UK
dc.date.accessioned2014-11-06T23:40:44Z-
dc.date.available2014-11-06T23:40:44Z-
dc.date.issued2012-09-11en_UK
dc.identifier.other198en_UK
dc.identifier.urihttp://hdl.handle.net/1893/21212-
dc.description.abstractBackground: Changes in the production of proliferating cell nuclear antigen (PCNA), a 36 kd protein involved in protein synthesis, within intestinal epithelia can provide an early indication of deviations to normal functioning. Inhibition or stimulation of cell proliferation and PCNA can be determined through immunohistochemical staining of intestinal tissue. Changes in the expression of PCNA act as an early warning system of changes to the gut and this application has not been applied to the fields of aquatic parasitology and fish health. The current study set out to determine whether a population of wild brown trout, Salmo trutta trutta (L.) harbouring an infection of the acanthocephalan Dentitruncus truttae Sinzar, 1955 collected from Lake Piediluco in Central Italy also effected changes in the expression of PCNA. Methods: A total of 29 brown trout were investigated, 19 of which (i.e. 65.5%) were found to harbour acanthocephalans (5-320 worms fish-1). Histological sections of both uninfected and infected intestinal material were immunostained for PCNA. Results: The expression of PCNA was observed in the epithelial cells in the intestinal crypts and within the mast cells and fibroblasts in the submucosa layer which is consistent with its role in cell proliferation and DNA synthesis. The number of PCNA-positive cells in both the intestinal epithelium and the submucosa layer in regions close to the point of parasite attachment were significantly higher than the number observed in uninfected individuals and in infected individuals in zones at least 0.7 cm from the point of parasite attachment (ANOVA, p < 0.05). Conclusions: An infection of the acanthocephalan D. truttae within the intestinal tract of S. t. trutta effected a significant increase in the number of PCNA positive cells (mast cells and fibroblasts) at the site of parasite attachment when compared to the number of positive cells found in uninfected conspecifics and in tissue zones away from the point of parasite attachment.en_UK
dc.language.isoenen_UK
dc.publisherBioMed Centralen_UK
dc.relationDezfuli BS, Giari L, Lui A, Squerzanti S, Castaldelli G, Shinn A, Manera M & Lorenzoni M (2012) Proliferative cell nuclear antigen (PCNA) expression in the intestine of Salmo trutta trutta naturally infected with an acanthocephalan. Parasites and Vectors, 5 (1), Art. No.: 198. https://doi.org/10.1186/1756-3305-5-198en_UK
dc.rights© 2012 Dezfuli et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.en_UK
dc.rights.urihttp://creativecommons.org/licenses/by/2.0/en_UK
dc.subjectCell proliferationen_UK
dc.subjectImmunohistochemistryen_UK
dc.subjectFish intestineen_UK
dc.subjectEnteric helminthen_UK
dc.titleProliferative cell nuclear antigen (PCNA) expression in the intestine of Salmo trutta trutta naturally infected with an acanthocephalanen_UK
dc.typeJournal Articleen_UK
dc.identifier.doi10.1186/1756-3305-5-198en_UK
dc.citation.jtitleParasites and Vectorsen_UK
dc.citation.issn1756-3305en_UK
dc.citation.volume5en_UK
dc.citation.issue1en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusVoR - Version of Recorden_UK
dc.author.emaila.p.shinn@stir.ac.uken_UK
dc.contributor.affiliationUniversity of Ferraraen_UK
dc.contributor.affiliationUniversity of Ferraraen_UK
dc.contributor.affiliationUniversity of Ferraraen_UK
dc.contributor.affiliationUniversity of Ferraraen_UK
dc.contributor.affiliationUniversity of Ferraraen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationUniversity of Teramoen_UK
dc.contributor.affiliationUniversity of Perugiaen_UK
dc.identifier.isiWOS:000310216500001en_UK
dc.identifier.scopusid2-s2.0-84865964517en_UK
dc.identifier.wtid614400en_UK
dc.contributor.orcid0000-0002-5434-2685en_UK
dcterms.dateAccepted2012-09-11en_UK
dc.date.filedepositdate2014-11-06en_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionVoRen_UK
local.rioxx.authorDezfuli, Bahram S|en_UK
local.rioxx.authorGiari, Luisa|en_UK
local.rioxx.authorLui, Alice|en_UK
local.rioxx.authorSquerzanti, Samantha|en_UK
local.rioxx.authorCastaldelli, Giuseppe|en_UK
local.rioxx.authorShinn, Andrew|0000-0002-5434-2685en_UK
local.rioxx.authorManera, Maurizio|en_UK
local.rioxx.authorLorenzoni, Massimo|en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate2014-11-06en_UK
local.rioxx.licencehttp://creativecommons.org/licenses/by/2.0/|2014-11-06|en_UK
local.rioxx.filenameParasites and Vectors 2012.pdfen_UK
local.rioxx.filecount1en_UK
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