Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/21078
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Rapid detection of shrimp white spot syndrome virus by real time, isothermal recombinase polymerase amplification assay
Authors: Xia, Xiaoming
Yu, Yongxin
Weidmann, Manfred
Pan, Yingjie
Yan, Shuling
Wang, Yongjie
Contact Email: m.w.weidmann@stir.ac.uk
Issue Date: 2014
Publisher: Public Library of Science
Citation: Xia X, Yu Y, Weidmann M, Pan Y, Yan S & Wang Y (2014) Rapid detection of shrimp white spot syndrome virus by real time, isothermal recombinase polymerase amplification assay, PLoS ONE, 9 (8), Art. No.: e104667.
Abstract: White spot syndrome virus (WSSV) causes large economic losses to the shrimp aquaculture industry, and thus far there are no efficient therapeutic treatments available against this lethal virus. In this study, we present the development of a novel real time isothermal recombinase polymerase amplification (RPA) assay for WSSV detection on a small ESEQuant Tube Scanner device. The RPA sensitivity, specificity and rapidity were evaluated by using a plasmid standard as well as viral and shrimp genomic DNAs. Compared with qPCR, the RPA assay revealed more satisfactory performance. It reached a detection limit up to 10 molecules in 95% of cases as determined by probit analysis of 8 independent experiments within 6.41±0.17 min at 39°C. Consequently, this rapid RPA method has great application potential for field use or point of care diagnostics.
Type: Journal Article
URI: http://hdl.handle.net/1893/21078
DOI Link: http://dx.doi.org/10.1371/journal.pone.0104667
Rights: © 2014 Xia et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Affiliation: Shanghai Ocean University
Shanghai Ocean University
Aquaculture
Shanghai Ocean University
University of Gottingen, Georg-August University
Shanghai Ocean University

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