Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/21002
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Detection of infectious pancreatic necrosis virus (IPNV) from asymptomatic redbanded seabream, Pagrus auriga Valenciennes, and common seabream, Pagrus pagrus (L.), using a non-destructive procedure
Authors: Lopez, Jimena Benjamin
Garcia-Rosado, Esther
Infante, Carlos
Cano, Irene
Manchado, Manuel
Castro, Dolores
Borrego, Juan J
Alonso, Maria del Carmen
Contact Email: benjamin.lopez-jimena1@stir.ac.uk
Keywords: sparid fish
infectious pancreatic necrosis virus
carrier state
non-destructive procedure
hybridization
PCR methods
Issue Date: Apr-2010
Publisher: Wiley-Blackwell
Citation: Lopez Jimena B, Garcia-Rosado E, Infante C, Cano I, Manchado M, Castro D, Borrego JJ & Alonso MdC (2010) Detection of infectious pancreatic necrosis virus (IPNV) from asymptomatic redbanded seabream, Pagrus auriga Valenciennes, and common seabream, Pagrus pagrus (L.), using a non-destructive procedure, Journal of Fish Diseases, 33 (4), pp. 311-319.
Abstract: A non-destructive procedure based on nested RT-PCR and dot-blot hybridization has been developed for the detection of asymptomatic IPNV-carrier fish. The pair of primers designed for RT-PCR amplified a 599-bp fragment of the pVP2 region within the polyprotein gene, resulting in the detection of IPNV genotype III.1. The use of a nested RT-PCR allowed the amplification of IPNV genotypes III.1 and I.2. In addition, a 191-bp probe was designed for hybridization studies used in combination with the nested RT-PCR. The application of the nested RT-PCR to analyse blood samples from asymptomatic redbanded seabream, Pagrus auriga, and common seabream, P. pagrus, specimens showed a 53.1% and 77.8% prevalence of IPNV-carriers, respectively. The combination of nested RT-PCR and dot-blot hybridization increased the detection rates up to 100% for redbanded seabream and 94.4% for common seabream. Therefore, the protocol described in this study is highly sensitive and specific for the detection of IPNV in asymptomatic carrier fish, and, in addition, the results demonstrate the carrier state in two newly cultured sparid species in southern Spain.
Type: Journal Article
URI: http://hdl.handle.net/1893/21002
DOI Link: http://dx.doi.org/10.1111/j.1365-2761.2009.01123.x
Rights: The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.
Affiliation: Aquaculture
University of Malaga
Centro IFAPA, Spain
University of Malaga
Centro IFAPA, Spain
University of Malaga
University of Malaga
University of Malaga

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