Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/21000
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dc.contributor.authorJeffery, Kathryn Janeen_UK
dc.contributor.authorAbernethy, Katharineen_UK
dc.contributor.authorTutin, Caroline E Gen_UK
dc.contributor.authorBruford, Michael Wen_UK
dc.date.accessioned2014-09-04T00:21:22Z-
dc.date.available2014-09-04T00:21:22Zen_UK
dc.date.issued2007-06en_UK
dc.identifier.urihttp://hdl.handle.net/1893/21000-
dc.description.abstractNaturally shed hairs are an important source of genetic material for both conservation and forensics but are notoriously poor sources of DNA. DNA degradation in hair roots is caused by apoptosis as part of the cycle of hair growth and by autolysis in decomposing animals. Shed hairs are additionally exposed to degenerative environmental processes. However, genetic studies rarely examine hair root morphologies or refer to root growth phases prior to analysis, and detailed knowledge of the rapidity of DNA degradation amongst shed hairs is lacking. We examined the effects of biological and environmental processes on western lowland gorilla (Gorilla gorilla gorilla Savage and Wyman) hair roots with respect to morphological characteristics and polymerase chain reaction (PCR) success at eight nuclear loci. Root type frequencies indicate that gorilla body hairs may exhibit a longer telogen phase than human head hairs. All plucked hair root types amplified more efficiently than shed hairs, and only 41% of shed hairs had root types considered suitable for genotyping. Telogen hairs from fresh nests were four-fold more useful for genotyping if the roots were associated with translucent epithelial tissue, and preselection of these root types doubled the overall data-yield to 58%. Nest age correlated with root morphology and PCR success, and PCR success was almost halved after 3 days of exposure. Finally, an association between postmortem interval, root morphology, and PCR success was observed that was consistent with postmortem changes reported in human head hairs. � 2007 The Linnean Society of London, Biological Journal of the Linnean Society, 2007, 91, 281-294.en_UK
dc.language.isoenen_UK
dc.publisherWiley-Blackwell for the Linnean Societyen_UK
dc.relationJeffery KJ, Abernethy K, Tutin CEG & Bruford MW (2007) Biological and environmental degradation of gorilla hair and microsatellite amplification success. Biological Journal of the Linnean Society, 91 (2), pp. 281-294. https://doi.org/10.1111/j.1095-8312.2007.00792.xen_UK
dc.rightsThe publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.en_UK
dc.rights.urihttp://www.rioxx.net/licenses/under-embargo-all-rights-reserveden_UK
dc.subjectDNA degradationen_UK
dc.subjectdecompositionen_UK
dc.subjectgorilla nestsen_UK
dc.subjecthair root morphologyen_UK
dc.subjecthair growth cycleen_UK
dc.subjectnon-invasive genotypingen_UK
dc.titleBiological and environmental degradation of gorilla hair and microsatellite amplification successen_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate2999-12-31en_UK
dc.rights.embargoreason[Jeffery et al 2007. BJLS.v91.p281.pdf] The publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work.en_UK
dc.identifier.doi10.1111/j.1095-8312.2007.00792.xen_UK
dc.citation.jtitleBiological Journal of the Linnean Societyen_UK
dc.citation.issn1095-8312en_UK
dc.citation.issn0024-4066en_UK
dc.citation.volume91en_UK
dc.citation.issue2en_UK
dc.citation.spage281en_UK
dc.citation.epage294en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusVoR - Version of Recorden_UK
dc.author.emailk.a.abernethy@stir.ac.uken_UK
dc.contributor.affiliationBiological and Environmental Sciencesen_UK
dc.contributor.affiliationBiological and Environmental Sciencesen_UK
dc.contributor.affiliationUniversity of Stirlingen_UK
dc.contributor.affiliationCardiff Universityen_UK
dc.identifier.isiWOS:000246756400009en_UK
dc.identifier.scopusid2-s2.0-34447098750en_UK
dc.identifier.wtid792851en_UK
dc.contributor.orcid0000-0002-2632-0008en_UK
dc.contributor.orcid0000-0002-0393-9342en_UK
dcterms.dateAccepted2007-06-30en_UK
dc.date.filedepositdate2014-08-22en_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionVoRen_UK
local.rioxx.authorJeffery, Kathryn Jane|0000-0002-2632-0008en_UK
local.rioxx.authorAbernethy, Katharine|0000-0002-0393-9342en_UK
local.rioxx.authorTutin, Caroline E G|en_UK
local.rioxx.authorBruford, Michael W|en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate2999-12-31en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/under-embargo-all-rights-reserved||en_UK
local.rioxx.filenameJeffery et al 2007. BJLS.v91.p281.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source0024-4066en_UK
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