Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/20985
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: A combined RT-PCR and dot-blot hybridization method reveals the coexistence of SJNNV and RGNNV betanodavirus genotypes in wild meagre (Argyrosomus regius)
Authors: Lopez, Jimena Benjamin
Cherif, Nadia
Garcia-Rosado, Esther
Infante, Carlos
Cano, Irene
Castro, Dolores
Hammami, Salah
Borrego, Juan J
Alonso, Maria del Carmen
Contact Email: benjamin.lopez-jimena1@stir.ac.uk
Keywords: betanodavirus
degenerate primers
dot-blot hybridization
RGNNV
RT-PCR
SJNNV
Issue Date: Oct-2010
Publisher: Wiley-Blackwell
Citation: Lopez Jimena B, Cherif N, Garcia-Rosado E, Infante C, Cano I, Castro D, Hammami S, Borrego JJ & Alonso MdC (2010) A combined RT-PCR and dot-blot hybridization method reveals the coexistence of SJNNV and RGNNV betanodavirus genotypes in wild meagre (Argyrosomus regius), Journal of Applied Microbiology, 109 (4), pp. 1361-1369.
Abstract: Aims: To detect the possible coexistence of striped jack nervous necrosis virus (SJNNV) and red-spotted grouper nervous necrosis virus (RGNNV) genotypes in a single fish, a methodology based on the combination of PCR amplification and blot hybridization has been developed and applied in this study. Methods and Results: The degenerate primers designed for the PCR procedure target the T4 region within the capsid gene, resulting in the amplification of both genotypes. The subsequent hybridization of these amplification products with two different specific digoxigenin-labelled probes resulted in the identification of both genotypes separately. The application of the RT-PCR protocol to analyse blood samples from asymptomatic wild meagre (Argyrosomus regius) specimens has shown a 46·87% of viral nervous necrosis virus carriers. The combination of RT-PCR and blot hybridization increases the detection rate up to 90·62%, and, in addition, it has shown the coexistence of both genotypes in 18 out of the 32 specimens analysed (56·25%). Conclusions: This study reports the coexistence of betanodaviruses belonging to two different genotypes (SJNNV and RGNNV) in wild fish specimens. Significance and Impact of the Study: This is the first report demonstrating the presence of SJNNV and RGNNV genotypes in the same specimen. This study also demonstrates a carrier state in this fish species for the first time.
Type: Journal Article
URI: http://hdl.handle.net/1893/20985
DOI Link: http://dx.doi.org/10.1111/j.1365-2672.2010.04759.x
Rights: The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.
Affiliation: Aquaculture
Institute of Veterinary Research Tunisia
University of Malaga
Centro IFAPA, Spain
University of Malaga
University of Malaga
Institute of Veterinary Research Tunisia
University of Malaga
University of Malaga

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