Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/20876
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Implication of the mineralocorticoid axis in rainbow trout osmoregulation during salinity acclimation
Authors: Kiilerich, Pia
Milla, Sylvain
Sturm, Armin
Valotaire, Claudiane
Chevolleau, Sylvie
Giton, Franck
Terrien, Xavier
Fiet, Jean
Fostier, Alexis
Debrauwer, Laurent
Prunet, Patrick
Contact Email: armin.sturm@stir.ac.uk
Keywords: fish
osmoregulation
stress
cortisol
corticosteroid
mineralocorticoid
glucocorticoid
salinity
adrenal
hormone
Issue Date: May-2011
Publisher: Society for Endocrinology
Citation: Kiilerich P, Milla S, Sturm A, Valotaire C, Chevolleau S, Giton F, Terrien X, Fiet J, Fostier A, Debrauwer L & Prunet P (2011) Implication of the mineralocorticoid axis in rainbow trout osmoregulation during salinity acclimation, Journal of Endocrinology, 209 (2), pp. 221-235.
Abstract: Cortisol and glucocorticoid receptors (GRs) play an important role in fish osmoregulation, whereas the involvement of the mineralocorticoid receptor (MR) and its putative ligand 11-deoxycorticosterone (DOC) is poorly investigated. In this study, we assessed the implication of DOC and MR in rainbow trout (Oncorhynchus mykiss) osmoregulation during hypo- and hypersaline acclimation in parallel with the cortisol-GR system. A RIA for DOC was developed to measure plasma DOC levels, and a MR-specific antibody was developed to localize MR protein in the gill, intestine, and kidney. This is the first study to report DOC plasma levels during salinity change and MR localization in fish osmoregulatory tissue. Corticosteroid receptor mRNA abundance was investigated in osmoregulatory tissue during salinity acclimation, and the effect of cortisol and DOC on ionic transporters gene expression was assayed using an in vitro gill incubation method. Differential tissue-, salinity-, and time-dependent changes in MR mRNA levels during both hyper- and hyposaline acclimations and the ubiquitous localization of MR in osmoregulatory tissue suggest a role for the MR in osmoregulation. Presumably, DOC does not act as ligand for MR in osmoregulation because there were no changes in plasma DOC levels during either freshwater-seawater (FW-SW) or SW-FW acclimation or any effect of DOC on gill ionic transporter mRNA levels in the gill. Taken together, these results suggest a role for MR, but not for DOC, in osmoregulation and confirm the importance of cortisol as a major endocrine regulator of trout osmoregulation.
Type: Journal Article
URI: http://hdl.handle.net/1893/20876
DOI Link: http://dx.doi.org/10.1530/JOE-10-0371
Rights: The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.
Affiliation: Institut National de la Recherche Agronomique
Institut National de la Recherche Agronomique
Aquaculture
Institut National de la Recherche Agronomique
Institut National de la Recherche Agronomique
Hopital Henri Mondor, Faculte de Medecine
Institut National de la Recherche Agronomique
Hopital Henri Mondor, Faculte de Medecine
SCRIBE-INRA, France
Institut National de la Recherche Agronomique
Institut National de la Recherche Agronomique

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