Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/20874
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Stress and innate immunity in carp: Corticosteroid receptors and pro-inflammatory cytokines
Authors: Stolte, Ellen H
Nabuurs, Sander B
Bury, Nicholas R
Sturm, Armin
Flik, Gert
Savelkoul, Huub F J
Verburg-van, Kemenade B M Lidy
Contact Email: armin.sturm@stir.ac.uk
Keywords: GR
MR
Teleost
Alternative splicing
Immune system
Cytokine expression
Common carp
Issue Date: Nov-2008
Publisher: Elsevier
Citation: Stolte EH, Nabuurs SB, Bury NR, Sturm A, Flik G, Savelkoul HFJ & Verburg-van Kemenade BML (2008) Stress and innate immunity in carp: Corticosteroid receptors and pro-inflammatory cytokines, Molecular Immunology, 46 (1), pp. 70-79.
Abstract: The stress hormone cortisol is deeply involved in immune regulation in all vertebrates. Common carp (Cyprinus carpio L.) express four corticoid receptors that may modulate immune responses: three glucocorticoid receptors (GR); GR1, with two splice variants (GR1a and GR1b), GR2 and a single mineralocorticoid receptor (MR). All receptors are expressed as of 4 days post-fertilization and may thus play a critical role in development and functioning of the adult immune system. Immune tissues and cells predominantly express mRNA for GRs compared to mRNA for the MR. Three-dimensional protein structure modeling predicts, and transfection assays confirm that alternative splicing of GR1 does not influence the capacity to induce transcription of effector genes. When tested for cortisol activation, GR2 is the most sensitive corticoid receptor in carp, followed by the MR and GR1a and GR1b. Lipopolysacharide (LPS) treatment of head kidney phagocytes quickly induces GR1 expression and inhibits GR2 expression. Cortisol treatment in vivo enhances GR1a and MR mRNA expression, but only mildly, and cortisol treatment in vitro does not affect receptor expression of phagocytes. Cortisol has no direct effect on the LPS-induced receptor profile. Therefore, an immune rather than a stress stimulus regulates GR expression. Cortisol administered at stress levels to phagocytes in vitro significantly inhibits LPS-induced expression of the pro-inflammatory cytokines tumor necrosis factor alpha (TNF-α) and interleukin-12 (IL-12) (subunit p35) and of inducible nitric oxide synthase (iNOS) expression. A physiologically differential function for GR1 and GR2 in the immune response of fish to infection is indicated.
Type: Journal Article
URI: http://hdl.handle.net/1893/20874
DOI Link: http://dx.doi.org/10.1016/j.molimm.2008.07.022
Rights: The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.
Affiliation: Wageningen University
Radboud University Medical Centre Nijmegen
King's College London
Aquaculture
Radboud University Nijmegen
Wageningen University
Wageningen University

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