Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/20626
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Influence of dietary docosahexaenoic acid in combination with other long-chain polyunsaturated fatty acids on expression of biosynthesis genes and phospholipid fatty acid compositions in tissues of post-smolt Atlantic salmon (Salmo salar)
Authors: Betancor, Monica
Howarth, Fraser J E
Glencross, Brett
Tocher, Douglas R
Contact Email: d.r.tocher@stir.ac.uk
Keywords: ARA
Atlantic salmon
DHA
EPA
LC-PUFA biosynthesis
Issue Date: Jun-2014
Publisher: Elsevier
Citation: Betancor M, Howarth FJE, Glencross B & Tocher DR (2014) Influence of dietary docosahexaenoic acid in combination with other long-chain polyunsaturated fatty acids on expression of biosynthesis genes and phospholipid fatty acid compositions in tissues of post-smolt Atlantic salmon (Salmo salar), Comparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology, 172-173, pp. 74-89.
Abstract: To investigate interactions of dietary LC-PUFA, a dose-response study with a range of docosahexaenoic acid (DHA; 22:6n - 3) levels (1 g kg- 1, 5 g kg- 1, 10 g kg- 1, 15 g kg- 1 and 20 g kg- 1) was performed with post-smolts (111 ± 2.6 g; mean ± S.D.) over a nine-week feeding period. Additional diets included 10 g kg- 1 DHA in combination with 10 g kg- 1 of either eicosapentaenoic acid (EPA; 20:5n - 3) or arachidonic acid (ARA; 20:4n - 6), and a diet containing 5 g kg- 1 each of DHA and EPA. The liver, brain, head kidney and gill were collected at the conclusion of the trial, and lipid and fatty acid compositions were determined as well as expression of genes of LC-PUFA biosynthesis. Total lipid content and class composition were largely unaffected by changes in dietary LC-PUFA. However, phospholipid (PL) fatty acid compositions generally reflected that of the diet, although the response varied between tissues. The liver most strongly reflected diet, followed by the head kidney. In both tissues increasing dietary DHA led to significantly increased DHA in PL and inclusion of EPA or ARA led to higher levels of these fatty acids. The brain showed the most conserved composition and gene expression profile, with increased dietary LC-PUFA resulting in only minor changes in PL fatty acids. Dietary LC-PUFA significantly affected the expression of Δ6 and Δ5 desaturases, Elovl 2, 4 and 5, and SREBPs although this varied between tissues with greatest effects observed in the liver followed by the head kidney, similar to PL fatty acid compositions.
Type: Journal Article
URI: http://hdl.handle.net/1893/20626
DOI Link: http://dx.doi.org/10.1016/j.cbpb.2014.04.007
Rights: Published in Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology by Elsevier; Elsevier believes that individual authors should be able to distribute their accepted author manuscripts for their personal voluntary needs and interests, e.g. posting to their websites or their institution’s repository, e-mailing to colleagues. The Elsevier Policy is as follows: Authors retain the right to use the accepted author manuscript for personal use, internal institutional use and for permitted scholarly posting provided that these are not for purposes of commercial use or systematic distribution. An "accepted author manuscript" is the author’s version of the manuscript of an article that has been accepted for publication and which may include any author-incorporated changes suggested through the processes of submission processing, peer review, and editor-author communications.
Affiliation: Aquaculture
University of Stirling
Aquaculture
Aquaculture

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