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Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Genetic diversity in common carp stocks assayed by random-amplified polymorphic DNA markers
Authors: Basavaraju, Yaraguntappa
Prasad, Doddanajappa Theertha
Rani, Kumuda
Kumar, S Pradeep
Kumar, Umesha Dhakya Naika
Jahageerdar, Shrinivas
Srivastava, Prem Prakash
Penman, David
Mair, Graham C
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Keywords: Cyprinus carpio
random-amplified polymorphic DNA (RAPD)
Issue Date: 13-Feb-2007
Publisher: Wiley-Blackwell
Citation: Basavaraju Y, Prasad DT, Rani K, Kumar SP, Kumar UDN, Jahageerdar S, Srivastava PP, Penman D & Mair GC (2007) Genetic diversity in common carp stocks assayed by random-amplified polymorphic DNA markers, Aquaculture Research, 38 (2), pp. 147-155.
Abstract: The common carp (Cyprinus carpio L.) is one of the major aquaculture species, contributing nearly 35% to the inland fish production in Karnataka, India. Stocks collected from Hungary (2), Indonesia and Vietnam were assessed alongside two local stocks in a series of culture performance trials with the objective of setting up a base population for developing a breeding programme. The present study deals with the genetic divergence and polymorphism in these six stocks using random-amplified polymorphic DNA (RAPD) markers. A total of 180 decamer random primers were screened for polymerase chain reaction (PCR) amplification (OPA 1-20, OPB1-20, OPC1-20, OPD1-20, OPE1-20, OPF1-20, OPG1-20, OPP1-20 and OPM1-20). Eight primers were selected for analysis of common carp genotypes (OPA-7, OPA-20, OPB-17, OPF-10, OP F-9, OPG-4, OPG-9 and OPP-16). Out of 492 bands recorded, 57.1% were polymorphic. Stepwise regression analysis was carried out to find best combination markers affecting body weight (P < 0.001). The results demonstrate major differences in the genetic structures between different stocks. Dendrogram data showed grouping of individuals according to stocks and corresponding data variables revealed the per cent homology within the stock and also found markers correlating to the body weight
Type: Journal Article
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Rights: The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.
Affiliation: Animal and Fisheries University
University of Agricultural Sciences, India
Animal and Fisheries University
Animal and Fisheries University
Animal and Fisheries University
Central Institute of Fisheries Education
Central Institute of Fisheries Education
Flinders University

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