Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/19746
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Intraspecific diversity of Edwardsiella ictaluri isolates from diseased freshwater catfish, Pangasianodon hypophthalmus (Sauvage), cultured in the Mekong Delta, Vietnam
Authors: Bartie, Katie L
Austin, Frank W
Diab, Amer
Dickson, Cathryn
Dung, Tu Thanh
Giacomini, Mauro
Crumlish, Margaret
Contact Email: margaret.crumlish@stir.ac.uk
Keywords: Edwardsiella ictaluri
genotyping
Pangasianodon hypophthalmus
pulsed-field gel electrophoresis
Rep-PCR
Vietnam
Issue Date: Sep-2012
Publisher: Wiley-Blackwell
Citation: Bartie KL, Austin FW, Diab A, Dickson C, Dung TT, Giacomini M & Crumlish M (2012) Intraspecific diversity of Edwardsiella ictaluri isolates from diseased freshwater catfish, Pangasianodon hypophthalmus (Sauvage), cultured in the Mekong Delta, Vietnam, Journal of Fish Diseases, 35 (9), pp. 671-682.
Abstract: A molecular epidemiology study was conducted on 90 Edwardsiella ictaluri isolates recovered from diseased farmed freshwater catfish, Pangasianodon hypophthalmus, cultured in the Mekong Delta, Vietnam. Thirteen isolates of E. ictaluri derived from diseased channel catfish, Ictalurus punctatus, cultured in the USA were included for comparison. All the E. ictaluri isolates tested were found to be biochemically indistinguishable. A repetitive (rep)-PCR using the single (GTG)5 primer was shown to possess limited discriminatory power, yielding two similar DNA profiles categorized as (GTG)5-PCR group 1 or 2 among the Vietnam isolates and (GTG)5-PCR group 1 within the USA isolates. Macrorestriction analysis identified 14 and 22 unique pulsotypes by XbaI and SpeI, respectively, among a subset of 59 E. ictaluri isolates. Numerical analysis of the combined macrorestriction profiles revealed three main groups: a distinct cluster formed exclusively of the USA isolates, and a major and minor cluster with outliers contained the Vietnam isolates. Antibiotic susceptibility and plasmid profiling supported the existence of the three groups. The results indicate that macrorestriction analysis may be regarded as a suitable typing method among the E. ictaluri species of limited intraspecific diversity. Furthermore, the findings suggest that E. ictaluri originating from Vietnam may constitute a distinct genetic group.
Type: Journal Article
URI: http://hdl.handle.net/1893/19746
DOI Link: http://dx.doi.org/10.1111/j.1365-2761.2012.01376.x
Rights: The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.
Affiliation: University of Stirling
Mississippi State University
Aquaculture
Aquaculture
College of Agriculture, Can Tho
University of Genoa
Aquaculture

Files in This Item:
File Description SizeFormat 
J of FD 2012.pdf340.64 kBAdobe PDFUnder Embargo until 31/12/2999     Request a copy

Note: If any of the files in this item are currently embargoed, you can request a copy directly from the author by clicking the padlock icon above. However, this facility is dependant on the depositor still being contactable at their original email address.

This item is protected by original copyright



Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

If you believe that any material held in STORRE infringes copyright, please contact library@stir.ac.uk providing details and we will remove the Work from public display in STORRE and investigate your claim.