Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/19725
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: A portable reverse transcription recombinase polymerase amplification assay for rapid detection of foot-and-mouth disease virus
Authors: El, Wahed Ahmed Abd
El-Deeb, Ayman
El-Tholoth, Mohamed
El, Kader Hanaa Abd
Ahmed, Abeer
Hassan, Sayed
Hoffmann, Bernd
Haas, Bernd
Shalaby, Mohamed A
Hufert, Frank T
Weidmann, Manfred
Contact Email: m.w.weidmann@stir.ac.uk
Issue Date: Aug-2013
Publisher: Public Library of Science
Citation: El Wahed AA, El-Deeb A, El-Tholoth M, El Kader HA, Ahmed A, Hassan S, Hoffmann B, Haas B, Shalaby MA, Hufert FT & Weidmann M (2013) A portable reverse transcription recombinase polymerase amplification assay for rapid detection of foot-and-mouth disease virus, PLoS ONE, 8 (8), Art. No.: e71642.
Abstract: Foot-and-mouth disease (FMD) is a trans-boundary viral disease of livestock, which causes huge economic losses and constitutes a serious infectious threat for livestock farming worldwide. Early diagnosis of FMD helps to diminish its impact by adequate outbreak management. In this study, we describe the development of a real-time reverse transcription recombinase polymerase amplification (RT-RPA) assay for the detection of FMD virus (FMDV). The FMDV RT-RPA design targeted the 3D gene of FMDV and a 260 nt molecular RNA standard was used for assay validation. The RT-RPA assay was fast (4-10 minutes) and the analytical sensitivity was determined at 1436 RNA molecules detected by probit regression analysis. The FMDV RT-RPA assay detected RNA prepared from all seven FMDV serotypes but did not detect classical swine fever virus or swine vesicular disease virus. The FMDV RT-RPA assay was used in the field during the recent FMD outbreak in Egypt. In clinical samples, reverse transcription polymerase chain reaction (RT-PCR) and RT-RPA showed a diagnostic sensitivity of 100% and 98%, respectively. In conclusion, FMDV RT-RPA was quicker and much easier to handle in the field than real-time RT-PCR. Thus RT-RPA could be easily implemented to perform diagnostics at quarantine stations or farms for rapid spot-of-infection detection.
Type: Journal Article
URI: http://hdl.handle.net/1893/19725
DOI Link: http://dx.doi.org/10.1371/journal.pone.0071642
Rights: © 2013 Abd El Wahed et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Affiliation: Mansoura University
Cairo University
Mansoura University
Animal Health Research Institute (Egypt)
Animal Health Research Institute (Egypt)
Animal Health Research Institute (Egypt)
Federal Research Institute for Animal Health
Federal Research Institute for Animal Health
Cairo University
University of Gottingen, Georg-August University
Aquaculture

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