|Appears in Collections:||Aquaculture Journal Articles|
|Peer Review Status:||Refereed|
|Title:||Enrichment of Artemia nauplii in vitamin A, vitamin C and methionine using liposomes|
Navarro, Juan Carlos
|Citation:||Monroig O, Navarro JC, Amat F & Hontoria F (2007) Enrichment of Artemia nauplii in vitamin A, vitamin C and methionine using liposomes, Aquaculture, 269 (1-4), pp. 504-513.|
|Abstract:||Several types of liposomes were used to enrich Artemia nauplii in vitamin A, vitamin C and free methionine. In a first experiment, unilamellar liposomes formulated with krill phospholipid extract and retinyl palmitate demonstrated their capability to enhance the retinol content of Artemia nauplii. Furthermore, the increase in retinol was related to the amount of retinyl palmitate included in the liposomes as vitamin A source. These findings yield the possibility of using such vesicles to bioencapsulate simultaneously both vitamin A and essential fatty acids present in the krill phospholipid extract. A second enrichment was carried out with unilamellar liposomes composed of soybean phosphatidylcholine and loaded with sodium ascorbate as vitamin C source. Our results did not show that vitamin C content in the nauplii could be increased using unilamellar liposomes. This was most likely due to the degradation of the vitamin C during enrichment as well as the ascorbate leakage. Finally, a third experiment assessed enrichment in free methionine using liposomes of different lamellarity (unilamellar or multilamellar) and composed of either soybean phosphatidylcholine or dipalmitoyl phosphatidylcholine, both combined with cholesterol as a membrane stabilizer. Results indicated that multilamellar liposomes represent a useful tool to deliver methionine to Artemia nauplii. Enhanced protection given by their multiple bilayers in comparison to unilamellar liposomes could account for the higher ability displayed by multilamellar vesicles for free methionine bioencapsulation.|
|Rights:||Published in Aquaculture by Elsevier; Elsevier believes that individual authors should be able to distribute their accepted author manuscripts for their personal voluntary needs and interests, e.g. posting to their websites or their institution’s repository, e-mailing to colleagues. The Elsevier Policy is as follows: Authors retain the right to use the accepted author manuscript for personal use, internal institutional use and for permitted scholarly posting provided that these are not for purposes of commercial use or systematic distribution. An "accepted author manuscript" is the author’s version of the manuscript of an article that has been accepted for publication and which may include any author-incorporated changes suggested through the processes of submission processing, peer review, and editor-author communications.|
|Monroig vits (AQUA-S-06-01342).pdf||572.73 kB||Adobe PDF||View/Open|
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