Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/19632
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Molecular cloning, tissue distribution and daily expression of cry1 and cry2 clock genes in European seabass (Dicentrarchus labrax)
Authors: Del, Pozo Ana
Vera, LM
Sanchez, Jose A
Sanchez-Vazquez, F Javier
Contact Email: luisa.veraandujar@stir.ac.uk
Keywords: Circadian rhythm
cry
Cryptochrome
Molecular clock
Sea bass
Issue Date: Nov-2012
Publisher: Elsevier
Citation: Del Pozo A, Vera L, Sanchez JA & Sanchez-Vazquez FJ (2012) Molecular cloning, tissue distribution and daily expression of cry1 and cry2 clock genes in European seabass (Dicentrarchus labrax), Comparative Biochemistry and Physiology Part A: Molecular and Integrative Physiology, 163 (3-4), pp. 364-371.
Abstract: Biological rhythms are driven by circadian oscillators, which are ultimately controlled by the cyclic expression of clock genes. Cryptochromes (CRY), blue light photoreceptors, belong to the negative elements of the transcriptional feedback loop into the molecular clock. This paper describes the cloning and characterization of two cryptochromes (cry1 and 2) in European seabass, which is considered an interesting chronobiology model due to its dual (diurnal/nocturnal) behavior. The cloned cDNA fragments encoded for two proteins of 567 and 668 amino acids, which included the FAD-binding and the DNA-photolyase domains. Moreover, both proteins had a high homology with cryptochrome proteins (Cry) of other teleost fish. These cry1 and 2 genes were expressed in several tissues of seabass (brain, liver, heart, retina, muscle, spleen, gill and intestine). In addition, the daily expression of cry1 was rhythmic in brain, heart and liver with the acrophase around ZT 03:15 h (after the onset of lights). Similarly, the cry2 daily expression was rhythmic in liver, peaking at ZT 03:28 h, whereas in brain the acrophase was at ZT 11:08 h (shortly prior to the offset of lights). These findings provide new elements to help understanding the functioning of the molecular clock of seabass.
Type: Journal Article
URI: http://hdl.handle.net/1893/19632
DOI Link: http://dx.doi.org/10.1016/j.cbpa.2012.07.004
Rights: The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.
Affiliation: University of Murcia
Aquaculture
University of Murcia
University of Murcia

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