Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/19628
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dc.contributor.authorVera, LMen_UK
dc.contributor.authorMontoya, Anderen_UK
dc.contributor.authorSanchez-Vazquez, F Javieren_UK
dc.date.accessioned2014-03-28T23:31:48Z-
dc.date.available2014-03-28T23:31:48Zen_UK
dc.date.issued2013-02-17en_UK
dc.identifier.urihttp://hdl.handle.net/1893/19628-
dc.description.abstractFeeding time is a potent zeitgeber capable of synchronising behavioural and physiological daily rhythms in fish. However, the effect of feeding time on the daily rhythm of drugs toxicity and/or effectiveness remains unexplored to date. In this paper we investigated the day/night variations in the effectiveness of an anaesthetic commonly used in fish (Tricaine, MS-222) in a teleost of great chronobiological and aquaculture interest (gilthead seabream). To this end, fish were kept under LD 12:12 and fed at mid-light (ML), mid-darkness (MD) or random times (RD). The time needed to induce anaesthesia (reduction of locomotor activity) during MS-222 exposure (65 mg/L) as well as the recovery period were investigated at ML and MD in the three experimental groups using specialised video tracking software. In addition, daily rhythms of GST activity in the liver (as an indicator of detoxification processes) and plasma MS-222 concentration (related to uptake) were determined. The results revealed that MS-222 effectiveness in the ML group was higher during the day than at night (significant reduction of activity after 3 min vs. 5 min) whereas in the MD group, the daily variation of MS-222 effectiveness was inverted (significant reduction of activity after 7 min at ML vs. 2 min at MD), suggesting that feeding time can shift the day-night variations in the effectiveness of MS-222. Hepatic GST also seemed to be affected by feeding time: in fish fed at MD or RD this enzyme activity showed significant differences during the day, and the highest levels were found at different times of the day in each group. Plasma MS-222 concentrations were higher at ML (142.4±12.8 ng/ml) than at MD (96.3±10.9 ng/ml) (t-Student, p<0.05). These results suggest that the daily variation in MS-222 concentration following exposure might be involved, among other factors, in the existence of day-night variations in the effectiveness of this anaesthetic. Furthermore, manipulation of the feeding schedule can be used to modify the daily variations in MS-222 effectiveness, which has basic as well applied implications for optimising anaesthesia protocols in fish aquaculture.en_UK
dc.language.isoenen_UK
dc.publisherElsevieren_UK
dc.relationVera L, Montoya A & Sanchez-Vazquez FJ (2013) Effectiveness of the anaesthetic MS-222 in gilthead seabream, Sparus aurata: effect of feeding time and day-night variations in plasma MS-222 concentration and GST activity. Physiology and Behavior, 110-111, pp. 51-57. https://doi.org/10.1016/j.physbeh.2012.12.012en_UK
dc.rightsThe publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.en_UK
dc.rights.urihttp://www.rioxx.net/licenses/under-embargo-all-rights-reserveden_UK
dc.subjectSparus aurataen_UK
dc.subjectMS-222 effectivenessen_UK
dc.subjectAnaesthesiaen_UK
dc.subjectFeeding-timeen_UK
dc.subjectGSTen_UK
dc.titleEffectiveness of the anaesthetic MS-222 in gilthead seabream, Sparus aurata: effect of feeding time and day-night variations in plasma MS-222 concentration and GST activityen_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate2999-12-18en_UK
dc.rights.embargoreason[Physiol and Behav 2013.pdf] The publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work.en_UK
dc.identifier.doi10.1016/j.physbeh.2012.12.012en_UK
dc.identifier.pmid23291569en_UK
dc.citation.jtitlePhysiology and Behavioren_UK
dc.citation.issn0031-9384en_UK
dc.citation.volume110-111en_UK
dc.citation.spage51en_UK
dc.citation.epage57en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusVoR - Version of Recorden_UK
dc.author.emailluisa.veraandujar@stir.ac.uken_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationUniversity of Murciaen_UK
dc.contributor.affiliationUniversity of Murciaen_UK
dc.identifier.isiWOS:000318132100009en_UK
dc.identifier.scopusid2-s2.0-84872473380en_UK
dc.identifier.wtid648759en_UK
dc.contributor.orcid0000-0003-0999-055Xen_UK
dc.date.accepted2012-12-21en_UK
dcterms.dateAccepted2012-12-21en_UK
dc.date.filedepositdate2014-03-27en_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionVoRen_UK
local.rioxx.authorVera, LM|0000-0003-0999-055Xen_UK
local.rioxx.authorMontoya, Ander|en_UK
local.rioxx.authorSanchez-Vazquez, F Javier|en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate2999-12-18en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/under-embargo-all-rights-reserved||en_UK
local.rioxx.filenamePhysiol and Behav 2013.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source0031-9384en_UK
Appears in Collections:Aquaculture Journal Articles

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