Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/18556
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Confocal scanning laser microscopy with complementary 3D image analysis allows quantitative studies of functional state of ionoregulatory cells in the nile tilapia (Oreochromis niloticus) following salinity challenge
Authors: Fridman, Sophie
Rana, Kausik
Bron, James
Contact Email: j.e.bron@stir.ac.uk
Keywords: mitochondria-rich cells
teleosts
confocal microscopy
Issue Date: Apr-2013
Publisher: Wiley-Blackwell
Citation: Fridman S, Rana K & Bron J (2013) Confocal scanning laser microscopy with complementary 3D image analysis allows quantitative studies of functional state of ionoregulatory cells in the nile tilapia (Oreochromis niloticus) following salinity challenge, Microscopy Research and Technique, 76 (4), pp. 412-418.
Abstract: The development of a novel three-dimensional image analysis technique of stacks generated by confocal laser scanning microscopy is described allowing visualization of mitochondria-rich cells (MRCs) in the seawater-adapted Nile tilapia in relation to their spatial location. This method permits the assessment and classification of both active and nonactive MRCs based on the distance of the top of the immunopositive cell from the epithelial surface. In addition, this technique offers the potential for informative and quantitative studies, for example, densitometric and morphometric measurements based on MRC functional state. Confocal scanning laser microscopy used with triple staining whole-mount immunohistochemistry was used to detect integumental MRCs in the yolk-sac larvae tail of the Nile tilapia following transfer from freshwater to elevated salinities, that is, 12.5 and 20 ppt. Mean active MRC volume was always significantly larger and displayed a greater staining intensity (GLM; P<0.05) than nonactive MRCs. Following transfer, the percentage of active MRCs was seen to increase as did MRC volume (GLM; P<0.05). Microsc. Res. Tech., 2013. © 2013 Wiley Periodicals, Inc.
Type: Journal Article
URI: http://hdl.handle.net/1893/18556
DOI Link: http://dx.doi.org/10.1002/jemt.22181
Rights: The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.
Affiliation: Aquaculture
Aquaculture
Aquaculture

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