Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/18442
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Interferon-mediated host response in experimentally induced salmonid alphavirus 1 infection in Atlantic salmon (Salmo salar L.)
Authors: Herath, Tharangani
Thompson, Kimberly
Adams, Alexandra
Richards, Randolph
Contact Email: r.h.richards@stir.ac.uk
Keywords: Atlantic salmon
Salmonid alphavirus
Interferon I
Mx protein
Immunohistochemistry
qRT-PCR
Issue Date: Sep-2013
Publisher: Elsevier
Citation: Herath T, Thompson K, Adams A & Richards R (2013) Interferon-mediated host response in experimentally induced salmonid alphavirus 1 infection in Atlantic salmon (Salmo salar L.), Veterinary Immunology and Immunopathology, 155 (1-2), pp. 9-20.
Abstract: Salmonid alphavirus (SAV) infection in cultured salmonids causes severe economic losses across Europe. Immune protection and antiviral mechanisms of the host against SAV are poorly characterised in vivo. Analysis of immune gene expression in head kidney of Atlantic salmon (Salmo salar L.) experimentally infected with SAV 1, using a quantitative reverse transcription polymerase chain reaction (qRT-PCR), revealed rapid induction of interferon I (INF-I), interferon II (INF-II) and INF-I associated Mx genes in SAV 1 infected fish compared to control fish injected with tissue culture supernatant. Mx protein was found to be highly expressed in the heart and mucosal membranes of infected fish by immunohistochemistry (IHC). Interestingly, the pathological changes that were observed in the target tissues of the virus became visible some time after peak expression of genes associated with the INF-I-pathway in head kidney tissue. These findings suggest that a non-specific antiviral immune response is rapidly induced during the early stages of SAV infection in salmon.
Type: Journal Article
URI: http://hdl.handle.net/1893/18442
DOI Link: http://dx.doi.org/10.1016/j.vetimm.2013.05.011
Rights: The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.
Affiliation: Aquaculture
Aquaculture
Aquaculture
Aquaculture

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