Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/18311
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Recombinase Polymerase Amplification Assay for Rapid Detection of Francisella tularensis
Authors: Euler, Milena
Wang, Yongjie
Otto, Peter
Tomaso, Herbert
Escudero, Raquel
Anda, Pedro
Hufert, Frank T
Weidmann, Manfred
Contact Email: m.w.weidmann@stir.ac.uk
Issue Date: Jul-2012
Publisher: American Society for Microbiology
Citation: Euler M, Wang Y, Otto P, Tomaso H, Escudero R, Anda P, Hufert FT & Weidmann M (2012) Recombinase Polymerase Amplification Assay for Rapid Detection of Francisella tularensis, Journal of Clinical Microbiology, 50 (7), pp. 2234-2238.
Abstract: Several real-time PCR approaches to develop field detection for Francisella tularensis, the infectious agent causing tularemia, have been explored. We report the development of a novel qualitative real-time isothermal recombinase polymerase amplification (RPA) assay for use on a small ESEQuant Tube Scanner device. The analytical sensitivity and specificity were tested using a plasmid standard and DNA extracts from infected rabbit tissues. The assay showed a performance comparable to real-time PCR but reduced the assay time to 10 min. The rapid RPA method has great application potential for field use or point-of-care diagnostics.
Type: Journal Article
URI: http://hdl.handle.net/1893/18311
DOI Link: http://dx.doi.org/10.1128/JCM.06504-11
Rights: The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.
Affiliation: University of Gottingen, Georg-August University
University of Gottingen, Georg-August University
Federal Research Institute for Animal Health
Federal Research Institute for Animal Health
Carlos III Health Institute (Instituto de Salud Carlos III)
Carlos III Health Institute (Instituto de Salud Carlos III)
University of Gottingen, Georg-August University
Aquaculture

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