Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/18310
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Rapid detection of important human pathogenic Phleboviruses
Authors: Weidmann, Manfred
Sanchez-Seco, M Paz
Sall, Amadou A
Ly, Peinda Ogo
Thiongane, Yaya
Lo, Modou Moustapha
Schley, Heike
Hufert, Frank T
Contact Email: m.w.weidmann@stir.ac.uk
Keywords: bunyavirus
Phlebovirus
Taqman
real-time PCR
Toscana virus
Rift Valley Fever virus
lightcycler
smartcycler
Issue Date: Feb-2008
Publisher: Elsevier
Citation: Weidmann M, Sanchez-Seco MP, Sall AA, Ly PO, Thiongane Y, Lo MM, Schley H & Hufert FT (2008) Rapid detection of important human pathogenic Phleboviruses, Journal of Clinical Virology, 41 (2), pp. 138-142.
Abstract: Background: Rapid diagnostics are not available for several human pathogens in the genus Phlebovirus of the Bunyaviridae. Objectives: To develop RT-PCR assays for Sandfly Fever Sicilian virus (SFSV), Sandfly Fever Naples virus (SFNV), Toscana virus (TOSV) and Rift Valley Fever virus (RVFV). Study design: RNA standards were generated and used to test the performance of the assays. Results: A detection limit of 10-100 RNA molecules was determined for the SFSV, TOSV and RVFV assays. The sensitivity of the SFNV assay was not determined. The TOSV and the RVFV assays detected recent isolates from Spain and Africa, respectively. Conclusion: The assays should help to improve surveillance of pathogenic Phleboviruses.
Type: Journal Article
URI: http://hdl.handle.net/1893/18310
DOI Link: http://dx.doi.org/10.1016/j.jcv.2007.10.001
Rights: The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.
Affiliation: Aquaculture
Carlos III Health Institute (Instituto de Salud Carlos III)
Institut Pasteur de Dakar
Institut Pasteur de Dakar
Institute for Agricultural Research
University of Gottingen, Georg-August University
University of Gottingen, Georg-August University
University of Gottingen, Georg-August University

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