Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/18309
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Quantitative analysis of particles, genomes and infectious particles in supernatants of haemorrhagic fever virus cell cultures
Authors: Weidmann, Manfred
Sall, Amadou A
Manuguerra, Jean-Claude
Koivogui, Lamine
Adjami, Aime
Traore, Faye Fatou
Hedlund, Kjell-Olaf
Lindegren, Gunnel
Mirazimi, Ali
Contact Email: m.w.weidmann@stir.ac.uk
Issue Date: 24-Feb-2011
Publisher: BioMed Central Ltd.
Citation: Weidmann M, Sall AA, Manuguerra J, Koivogui L, Adjami A, Traore FF, Hedlund K, Lindegren G & Mirazimi A (2011) Quantitative analysis of particles, genomes and infectious particles in supernatants of haemorrhagic fever virus cell cultures, Virology Journal, 8, Art. No.: 81.
Abstract: Information on the replication of viral haemorrhagic fever viruses is not readily available and has never been analysed in a comparative approach. Here, we compared the cell culture growth characteristics of haemorrhagic fever viruses (HFV), of the Arenaviridae, Filoviridae, Bunyaviridae, and Flavivridae virus families by performing quantitative analysis of cell culture supernatants by (i) electron microscopy for the quantification of virus particles, (ii) quantitative real time PCR for the quantification of genomes, and (iii) determination of focus forming units by coating fluorescent antibodies to infected cell monolayers for the quantification of virus infectivity. The comparative analysis revealed that filovirus and RVFV replication results in a surplus of genomes but varying degrees of packaging efficiency and infectious particles. More efficient replication and packaging was observed for Lassa virus, and Dengue virus resulting in a better yield of infectious particles while, YFV turned out to be most efficient with only 4 particles inducing one FFU. For Crimean-Congo haemorrhagic fever virus (CCHFV) a surplus of empty shells was observed with only one in 24 particles equipped with a genome. The complete particles turned out to be extraordinarily infectious.
Type: Journal Article
URI: http://hdl.handle.net/1893/18309
DOI Link: http://dx.doi.org/10.1186/1743-422X-8-81
Rights: © 2011 Weidmann et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Affiliation: Aquaculture
Institut Pasteur de Dakar
Institut Pasteur
Gamal Abdel Nasser University of Conakry
Centers for Disease Control and Prevention (CDC)
Merieux Foundation
Swedish Institute for Infectious Disease Control
Swedish Institute for Infectious Disease Control
Swedish Institute for Infectious Disease Control

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