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Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/18269
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dc.contributor.authorWeidmann, Manfreden_UK
dc.contributor.authorFaye, Ousmaneen_UK
dc.contributor.authorFaye, Oumaren_UK
dc.contributor.authorKranaster, Ramonen_UK
dc.contributor.authorMarx, Andreasen_UK
dc.contributor.authorNunes, Marcio R Ten_UK
dc.contributor.authorVasconcelos, Pedro F Cen_UK
dc.contributor.authorHufert, Frank Ten_UK
dc.contributor.authorSall, Amadou Aen_UK
dc.date.accessioned2014-10-29T23:19:50Z-
dc.date.available2014-10-29T23:19:50Zen_UK
dc.date.issued2010-07en_UK
dc.identifier.urihttp://hdl.handle.net/1893/18269-
dc.description.abstractBackground: Real-time assays for Yellow fever virus (YFV) would help to improve acute diagnostics in outbreak investigations. Objectives: To develop a real-time assay for YFV able to detect African and South American strains. Study design:Three short probe (14-18 nt) formats were compared and a plasmid-transcribed RNA standard was used to test the performance of the assays. Additionally the new TaqM1 enzyme was tested. Results: A locked nucleotide probe (LNA probe) performed best with an analytical sensitivity of 10 RNA molecules detected. 44 African and 10 South American strains were detectable. One South American strain from 1984 had a one-nucleotide deviation in the hybridisation sequence for which the LNA probe had to be adapted. Comparison of enzymes revealed that not all enzymes are suitable for LNA probes. Conclusion: The developed LNA probe based YFV real-time PCR performed best in an enzyme mix and less efficient using multifunctional enzymes.en_UK
dc.language.isoenen_UK
dc.publisherElsevieren_UK
dc.relationWeidmann M, Faye O, Faye O, Kranaster R, Marx A, Nunes MRT, Vasconcelos PFC, Hufert FT & Sall AA (2010) Improved LNA probe-based assay for the detection of African and South American yellow fever virus strains. Journal of Clinical Virology, 48 (3), pp. 187-192. https://doi.org/10.1016/j.jcv.2010.04.013en_UK
dc.rightsThe publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.en_UK
dc.rights.urihttp://www.rioxx.net/licenses/under-embargo-all-rights-reserveden_UK
dc.subjectYFVen_UK
dc.subjectReal-time PCRen_UK
dc.subjectLNA probeen_UK
dc.subjectMGB probeen_UK
dc.subjectEclipse probeen_UK
dc.subjectTaqM1 enzymeen_UK
dc.titleImproved LNA probe-based assay for the detection of African and South American yellow fever virus strainsen_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate3000-01-01en_UK
dc.rights.embargoreason[JCV 2010.pdf] The publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work.en_UK
dc.identifier.doi10.1016/j.jcv.2010.04.013en_UK
dc.citation.jtitleJournal of Clinical Virologyen_UK
dc.citation.issn1386-6532en_UK
dc.citation.volume48en_UK
dc.citation.issue3en_UK
dc.citation.spage187en_UK
dc.citation.epage192en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusVoR - Version of Recorden_UK
dc.author.emailm.w.weidmann@stir.ac.uken_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitut Pasteur de Dakaren_UK
dc.contributor.affiliationInstitut Pasteur de Dakaren_UK
dc.contributor.affiliationUniversity of Konstanzen_UK
dc.contributor.affiliationUniversity of Konstanzen_UK
dc.contributor.affiliationEvandro Chagas Instituteen_UK
dc.contributor.affiliationEvandro Chagas Instituteen_UK
dc.contributor.affiliationGeorg-August University Gottingenen_UK
dc.contributor.affiliationInstitut Pasteur de Dakaren_UK
dc.identifier.isiWOS:000278658600007en_UK
dc.identifier.scopusid2-s2.0-77953120432en_UK
dc.identifier.wtid675327en_UK
dc.contributor.orcid0000-0002-7063-7491en_UK
dcterms.dateAccepted2010-07-31en_UK
dc.date.filedepositdate2014-01-06en_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionVoRen_UK
local.rioxx.authorWeidmann, Manfred|0000-0002-7063-7491en_UK
local.rioxx.authorFaye, Ousmane|en_UK
local.rioxx.authorFaye, Oumar|en_UK
local.rioxx.authorKranaster, Ramon|en_UK
local.rioxx.authorMarx, Andreas|en_UK
local.rioxx.authorNunes, Marcio R T|en_UK
local.rioxx.authorVasconcelos, Pedro F C|en_UK
local.rioxx.authorHufert, Frank T|en_UK
local.rioxx.authorSall, Amadou A|en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate3000-01-01en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/under-embargo-all-rights-reserved||en_UK
local.rioxx.filenameJCV 2010.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source1386-6532en_UK
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