STORRE
STORRE: Stirling Online Research Repository
http://hdl.handle.net/1893/18265| Appears in Collections: | Aquaculture Journal Articles |
| Peer Review Status: | Refereed |
| Title: | Development of a Flow-Trough Microarray based Reverse Transcriptase Multiplex Ligation-Dependent Probe Amplification Assay for the Detection of European Bunyaviruses |
| Author(s): | Hasib, Lekbira Dilcher, Meik Hufert, Frank T Meyer-Koenig, Ursula Weidmann, Manfred |
| Contact Email: | m.w.weidmann@stir.ac.uk |
| Keywords: | European Bunyaviruses MLPA Flow-through chip Aseptic meningo-encephalitis |
| Issue Date: | Oct-2011 |
| Date Deposited: | 13-Jan-2014 |
| Citation: | Hasib L, Dilcher M, Hufert FT, Meyer-Koenig U & Weidmann M (2011) Development of a Flow-Trough Microarray based Reverse Transcriptase Multiplex Ligation-Dependent Probe Amplification Assay for the Detection of European Bunyaviruses. Molecular Biotechnology, 49 (2), pp. 176-186. https://doi.org/10.1007/s12033-011-9389-3 |
| Abstract: | It is suspected that apart from tick-borne encephalitis virus several additional European Arboviruses such as the sandfly borne Toscana virus, sandfly fever Sicilian virus and sandfly fever Naples virus, mosquito-borne Tahyna virus, Inkoo virus, Batai virus and tick-borne Uukuniemi virus cause aseptic meningo-encephalitis or febrile disease in Europe. Currently, the microarray technology is developing rapidly and there are many efforts to apply it to infectious diseases diagnostics. In order to arrive at an assay system useful for high throughput analysis of samples from aseptic meningo-encephalitis cases the authors developed a combined multiplex ligation-dependent probe amplification and flow-through microarray assay for the detection of European Bunyaviruses. These results show that this combined assay indeed is highly sensitive, and specific for the accurate detection of multiple viruses. |
| DOI Link: | 10.1007/s12033-011-9389-3 |
| Rights: | The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. |
| Licence URL(s): | http://www.rioxx.net/licenses/under-embargo-all-rights-reserved |
| File | Description | Size | Format | |
|---|---|---|---|---|
| Molecular Biotechnology 2011.pdf | Fulltext - Published Version | 1.94 MB | Adobe PDF | Under Embargo until 3000-01-01 Request a copy |
Note: If any of the files in this item are currently embargoed, you can request a copy directly from the author by clicking the padlock icon above. However, this facility is dependent on the depositor still being contactable at their original email address.
This item is protected by original copyright |
Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.
The metadata of the records in the Repository are available under the CC0 public domain dedication: No Rights Reserved https://creativecommons.org/publicdomain/zero/1.0/
If you believe that any material held in STORRE infringes copyright, please contact library@stir.ac.uk providing details and we will remove the Work from public display in STORRE and investigate your claim.