Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/18265
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Development of a Flow-Trough Microarray based Reverse Transcriptase Multiplex Ligation-Dependent Probe Amplification Assay for the Detection of European Bunyaviruses
Authors: Hasib, Lekbira
Dilcher, Meik
Hufert, Frank T
Meyer-Koenig, Ursula
Weidmann, Manfred
Contact Email: m.w.weidmann@stir.ac.uk
Keywords: European Bunyaviruses
MLPA
Flow-through chip
Aseptic meningo-encephalitis
Issue Date: Oct-2011
Publisher: Springer
Citation: Hasib L, Dilcher M, Hufert FT, Meyer-Koenig U & Weidmann M (2011) Development of a Flow-Trough Microarray based Reverse Transcriptase Multiplex Ligation-Dependent Probe Amplification Assay for the Detection of European Bunyaviruses, Molecular Biotechnology, 49 (2), pp. 176-186.
Abstract: It is suspected that apart from tick-borne encephalitis virus several additional European Arboviruses such as the sandfly borne Toscana virus, sandfly fever Sicilian virus and sandfly fever Naples virus, mosquito-borne Tahyna virus, Inkoo virus, Batai virus and tick-borne Uukuniemi virus cause aseptic meningo-encephalitis or febrile disease in Europe. Currently, the microarray technology is developing rapidly and there are many efforts to apply it to infectious diseases diagnostics. In order to arrive at an assay system useful for high throughput analysis of samples from aseptic meningo-encephalitis cases the authors developed a combined multiplex ligation-dependent probe amplification and flow-through microarray assay for the detection of European Bunyaviruses. These results show that this combined assay indeed is highly sensitive, and specific for the accurate detection of multiple viruses.
Type: Journal Article
URI: http://hdl.handle.net/1893/18265
DOI Link: http://dx.doi.org/10.1007/s12033-011-9389-3
Rights: The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.
Affiliation: University of Gottingen, Georg-August University
University of Gottingen, Georg-August University
University of Gottingen, Georg-August University
Albert Ludwigs University of Freiburg
Aquaculture

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