Please use this identifier to cite or link to this item:
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Advanced Yellow Fever Virus Genome Detection in Point-of-Care Facilities and Reference Laboratories
Authors: Domingo, Cristina
Patel, Pranav
Yillah, Jasmin
Weidmann, Manfred
Mendez, Jairo A
Nakoune, Emmanuel R
Niedrig, Matthias
Contact Email:
Issue Date: Dec-2012
Publisher: American Society for Microbiology
Citation: Domingo C, Patel P, Yillah J, Weidmann M, Mendez JA, Nakoune ER & Niedrig M (2012) Advanced Yellow Fever Virus Genome Detection in Point-of-Care Facilities and Reference Laboratories, Journal of Clinical Microbiology, 50 (12), pp. 4054-4060.
Abstract: Reported methods for the detection of the yellow fever viral genome are beset by limitations in sensitivity, specificity, strain detection spectra, and suitability to laboratories with simple infrastructure in areas of endemicity. We describe the development of two different approaches affording sensitive and specific detection of the yellow fever genome: a real-time reverse transcription-quantitative PCR (RT-qPCR) and an isothermal protocol employing the same primer-probe set but based on helicase-dependent amplification technology (RT-tHDA). Both assays were evaluated using yellow fever cell culture supernatants as well as spiked and clinical samples. We demonstrate reliable detection by both assays of different strains of yellow fever virus with improved sensitivity and specificity. The RT-qPCR assay is a powerful tool for reference or diagnostic laboratories with real-time PCR capability, while the isothermal RT-tHDA assay represents a useful alternative to earlier amplification techniques for the molecular diagnosis of yellow fever by field or point-of-care laboratories.
Type: Journal Article
DOI Link:
Rights: The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.
Affiliation: Robert Koch Institute
Robert Koch Institute
Robert Koch Institute
National Institute of Health Colombia
Pasteur Institute of Bangui
Robert Koch Institute

Files in This Item:
File Description SizeFormat 
Journal of Clinical Microbiology 2012.full.pdf726.62 kBAdobe PDFUnder Embargo until 31/12/2999     Request a copy

Note: If any of the files in this item are currently embargoed, you can request a copy directly from the author by clicking the padlock icon above. However, this facility is dependant on the depositor still being contactable at their original email address.

This item is protected by original copyright

Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

If you believe that any material held in STORRE infringes copyright, please contact providing details and we will remove the Work from public display in STORRE and investigate your claim.