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Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Differential Gene Expression During Smoltification of Atlantic Salmon (Salmo salar L.): a First Large-Scale Microarray Study
Authors: Seear, Paul J
Carmichael, Stephen N
Talbot, Richard T
Taggart, John
Bron, James
Sweeney, Glen E
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Keywords: Transcriptomics
Issue Date: Apr-2010
Publisher: Springer
Citation: Seear PJ, Carmichael SN, Talbot RT, Taggart J, Bron J & Sweeney GE (2010) Differential Gene Expression During Smoltification of Atlantic Salmon (Salmo salar L.): a First Large-Scale Microarray Study, Marine Biotechnology, 12 (2), pp. 126-140.
Abstract: The life cycle of the Atlantic salmon (Salmo salar) involves a period of 1 to 3 years in freshwater followed by migration to the sea where the salmon undergoes rapid growth. In preparation for the marine environment, while still in freshwater, the salmon undergo a transformation from a freshwater dwelling parr to a saltwater adapted smolt, a process known as smoltification. The Atlantic salmon Transcriptome Analysis of Important Traits of Salmon/Salmon Genome Project (TRAITS/SGP) cDNA microarray was used to investigate how gene expression alters during smoltification. Genes differentially expressed during smoltification were identified by comparing gene expression profiles in smolt brain, gill, and kidney tissue samples with those of parr. Of the three tissues investigated, the number of differentially expressed genes was the greatest in gill. Many of the differentially expressed genes could be assigned to one of four main categories: growth, metabolism, oxygen transport, and osmoregulation. Quantitative polymerase chain reaction successfully confirmed the differential expression of seven of the upregulated genes. The TRAITS/SGP cDNA microarray was used to successfully demonstrate for the first time how gene expression mediates smoltification in the Atlantic salmon. Changes in gene expression observed in this study reflected the physiological and biochemical changes recorded by previous studies describing the parr-smolt transformation. This study significantly increases our knowledge of smoltification and will benefit future studies in this area of research.
Type: Journal Article
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Rights: The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.
Affiliation: Cardiff University
University of Edinburgh
Roslin Institute
Cardiff University

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