Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/1815
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dc.contributor.authorBogevik, Andre Sen_UK
dc.contributor.authorOlsen, Rolf Een_UK
dc.contributor.authorTocher, Douglas Ren_UK
dc.date.accessioned2013-06-09T07:02:47Z-
dc.date.available2013-06-09T07:02:47Z-
dc.date.issued2008-06en_UK
dc.identifier.urihttp://hdl.handle.net/1893/1815-
dc.description.abstractThe substitution of fish oil with wax ester-rich calanoid copepod-derived oil in diets for carnivorous fish, such as Atlantic salmon, has previously indicated a lower lipid digestibility. This suggests that the fatty alcohols (FAlc) present in wax esters may be a poorer substrate for intestinal enzymes than the fatty acids (FA) in triacylglycerol, the major lipid in fish oil. The hypothesis tested was that the possible lower utilization of dietary FAlc by salmon enterocytes is at the level of uptake and that subsequent intracellular metabolism was identical to that of FA. A dual-labelled FAlc-FA metabolism assay was employed to determine simultaneous FAlc and FA uptake and relative utilisation in enterocytes isolated from pyloric caeca of Atlantic salmon fed either a diet supplemented with fish oil or wax ester-rich Calanus oil. The diets were fed for 10 weeks before caecal enterocytes from each dietary group were isolated and incubated with equimolar mixtures of either [1-14C]16:0 FA and [9,10(n)-3H]16:0 FAlc, or [1-14C]18:1n-9 FA and [9,10(n)-3H]18:1n-9 FAlc. Uptake was measured after 2 h with relative utilization of labelled FAlc and FA calculated as a percentage of uptake. Differences in uptake were observed, with FA showing higher uptake than FAlc, and 18:1 chains a higher uptake than 16:0. A proportion of unesterified FAlc was possibly recovered in the cells, but the majority of FALc was recovered in lipid classes such as triacylglycerol and phospholipids indicating substantial conversion of FAlc to FA followed by esterification. However, incorporation of FA and FAlc into esterified lipids was higher when derived from FA than from FAlc. Twenty-five to fifty percentage of the absorbed 16:0 FA was recovered in TAG fraction of the enterocytes compared with fifteen to seventy-five percentage of 18:1 FA. Twenty to thirty percentage of the absorbed 16:0 FA was recovered in the PC fraction of the enterocytes compared with only five to fifteen percentage of the 18:1 FA. Less than 15% of the fatty chains taken up by the cells was used for energy production, with significantly higher oxidation of 18:1 in enterocytes from fish fed the fish oil diet compared to the Calanus oil diet. However, overall, dietary copepod oil had little effect on FAlc and FA metabolism. Metabolic modification by elongation and/or desaturation was generally low at 1-5% of uptake. We conclude that our hypothesis was generally proved in that the uptake of FAlc by salmon enterocytes was lower than the uptake of FA and that subsequent intracellular metabolism of FAlc was similar to that of FA. However, unesterified FAlc was possibly recovered in the cells suggesting that the conversion to FA may not be concomitant with uptake.en_UK
dc.language.isoenen_UK
dc.publisherWiley-Blackwellen_UK
dc.relationBogevik AS, Olsen RE & Tocher DR (2008) Utilization and metabolism of palmityl and oleoyl fatty acids and alcohols in caecal enterocytes of Atlantic salmon (Salmo salar L.). Aquaculture Nutrition, 14 (3), pp. 270-280. https://doi.org/10.1111/j.1365-2095.2007.00528.xen_UK
dc.rightsPublished in Aquaculture Nutrition. Copyright: Wiley-Blackwell. The definitive version is available at www3.interscience.wiley.com; Rights as indicated by the Exclusive Licence Form: http://www.blackwellpublishing.com/pdf/anu_elf.pdfen_UK
dc.subjectenterocytesen_UK
dc.subjectAtlantic salmonen_UK
dc.subjectfatty aciden_UK
dc.subjectfatty alcoholen_UK
dc.subjectoxidationen_UK
dc.subjectesterificationen_UK
dc.subjectwax esteren_UK
dc.subjectmetabolismen_UK
dc.subjectAtlantic salmonen_UK
dc.subjectFishes Feeding and feedsen_UK
dc.subjectFishes Nutritionen_UK
dc.subjectCopepodaen_UK
dc.titleUtilization and metabolism of palmityl and oleoyl fatty acids and alcohols in caecal enterocytes of Atlantic salmon (Salmo salar L.)en_UK
dc.typeJournal Articleen_UK
dc.identifier.doi10.1111/j.1365-2095.2007.00528.xen_UK
dc.citation.jtitleAquaculture Nutritionen_UK
dc.citation.issn1365-2095en_UK
dc.citation.issn1353-5773en_UK
dc.citation.volume14en_UK
dc.citation.issue3en_UK
dc.citation.spage270en_UK
dc.citation.epage280en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusAM - Accepted Manuscripten_UK
dc.author.emaildrt1@stir.ac.uken_UK
dc.citation.date22/11/2007en_UK
dc.contributor.affiliationMatre Aquaculture Research Stationen_UK
dc.contributor.affiliationMatre Aquaculture Research Stationen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.identifier.isiWOS:000255463100010en_UK
dc.identifier.scopusid2-s2.0-43049086307en_UK
dc.identifier.wtid838318en_UK
dc.contributor.orcid0000-0002-8603-9410en_UK
dcterms.dateAccepted2007-11-22en_UK
dc.date.filedepositdate2009-11-20en_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionAMen_UK
local.rioxx.authorBogevik, Andre S|en_UK
local.rioxx.authorOlsen, Rolf E|en_UK
local.rioxx.authorTocher, Douglas R|0000-0002-8603-9410en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate2009-11-20en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/all-rights-reserved|2009-11-20|en_UK
local.rioxx.filenameFAlc enterocyte metab paper revised.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source1353-5773en_UK
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