Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/17945
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dc.contributor.authorLeclercq, Ericen_UK
dc.contributor.authorAntoni, Lucaen_UK
dc.contributor.authorBardon-Albaret, Agnesen_UK
dc.contributor.authorAnderson, Corey Ren_UK
dc.contributor.authorSomerset, Carly Ren_UK
dc.contributor.authorSaillant, Eric Aen_UK
dc.date.accessioned2014-08-18T23:11:09Z-
dc.date.available2014-08-18T23:11:09Zen_UK
dc.date.issued2014-07en_UK
dc.identifier.urihttp://hdl.handle.net/1893/17945-
dc.description.abstractThe objective of this study was to optimize the methodology for spectrophotometric determination of sperm concentration in Atlantic croaker Micropogonias undulatus L. milt and to estimate its potential for short-term cold-storage. The spectrophotometric determination of sperm concentration was evaluated using milt samples from six males serially diluted in Hank's balanced salt solution at 200 mOsm kg 1 (HBSS). The predictive power of regression models between sperm concentration and absorbance was determined from 200 to 500 nm and found to be highest within the visible spectrum despite a peak of milt absorbance at 288 nm. Absorbance reading at 400 nm was selected for further analysis to maximize the absorbance of the sample hence the sensitivity of the method while minimizing the impact of potential sample contamination with blood. The standard-curve of correlation between sperm absorbance at 400 nm and concentration was validated and held an accuracy ranging between 7.40% and +4.56% across males. Total sperm motility duration and the proportion of motile spermatozoa were significantly higher in milt samples diluted 1:3 in HBSS than in the undiluted control during up to 30 h of cold-storage.The methodologies investigated in this study can be applied to optimize sperm usage and achieve predictable artificial fertilization protocols in Atlantic croaker.en_UK
dc.language.isoenen_UK
dc.publisherAquaculture Researchen_UK
dc.relationLeclercq E, Antoni L, Bardon-Albaret A, Anderson CR, Somerset CR & Saillant EA (2014) Spectrophotometric determination of sperm concentration and short-term cold-storage of sperm in Atlantic croaker Micropogonias undulatus L. broodstock [Atlantic croaker milt management in hatchery]. Aquaculture Research, 45 (8), pp. 1283-1294. https://doi.org/10.1111/are.12073en_UK
dc.rightsThe publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.en_UK
dc.rights.urihttp://www.rioxx.net/licenses/under-embargo-all-rights-reserveden_UK
dc.subjectAtlantic croakeren_UK
dc.subjectsperm concentrationen_UK
dc.subjectspectrophotometryen_UK
dc.subjectcold storageen_UK
dc.subjectmotilityen_UK
dc.titleSpectrophotometric determination of sperm concentration and short-term cold-storage of sperm in Atlantic croaker Micropogonias undulatus L. broodstocken_UK
dc.title.alternativeAtlantic croaker milt management in hatcheryen_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate2999-12-22en_UK
dc.rights.embargoreason[LeclercqetalCroakerSpermStore2014.pdf] The publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work.en_UK
dc.identifier.doi10.1111/are.12073en_UK
dc.citation.jtitleAquaculture Researchen_UK
dc.citation.issn1365-2109en_UK
dc.citation.issn1355-557Xen_UK
dc.citation.volume45en_UK
dc.citation.issue8en_UK
dc.citation.spage1283en_UK
dc.citation.epage1294en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusVoR - Version of Recorden_UK
dc.author.emaileric.leclercq@stir.ac.uken_UK
dc.citation.date21/11/2012en_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationThe University of Southern Mississippien_UK
dc.contributor.affiliationThe University of Southern Mississippien_UK
dc.contributor.affiliationThe University of Southern Mississippien_UK
dc.contributor.affiliationThe University of Southern Mississippien_UK
dc.contributor.affiliationThe University of Southern Mississippien_UK
dc.identifier.isiWOS:000339275400002en_UK
dc.identifier.scopusid2-s2.0-84869864471en_UK
dc.identifier.wtid661254en_UK
dc.date.accepted2012-11-15en_UK
dcterms.dateAccepted2012-11-15en_UK
dc.date.filedepositdate2013-12-20en_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionVoRen_UK
local.rioxx.authorLeclercq, Eric|en_UK
local.rioxx.authorAntoni, Luca|en_UK
local.rioxx.authorBardon-Albaret, Agnes|en_UK
local.rioxx.authorAnderson, Corey R|en_UK
local.rioxx.authorSomerset, Carly R|en_UK
local.rioxx.authorSaillant, Eric A|en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate2999-12-22en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/under-embargo-all-rights-reserved||en_UK
local.rioxx.filenameLeclercqetalCroakerSpermStore2014.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source1355-557Xen_UK
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