Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/17943
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: MRSA decolonization of cotton rat nares by a combination treatment comprising lysostaphin and the antimicrobial peptide ranalexin
Authors: Desbois, Andrew P
Sattar, Abdul
Graham, Shirley
Warn, Peter A
Coote, Peter J
Contact Email: andrew.desbois@stir.ac.uk
Keywords: antibacterials
combination
infection control
isoleucyl-tRNA synthetase
ileS
mupirocin
methicillin-resistant Staphylococcus aureus
nasal carriage
Issue Date: Nov-2013
Publisher: Oxford University Press
Citation: Desbois AP, Sattar A, Graham S, Warn PA & Coote PJ (2013) MRSA decolonization of cotton rat nares by a combination treatment comprising lysostaphin and the antimicrobial peptide ranalexin, Journal of Antimicrobial Chemotherapy, 68 (11), pp. 2569-2575.
Abstract: Objectives: To evaluate the in vivo effectiveness of a combination treatment containing ranalexin (a natural antimicrobial peptide) and lysostaphin (an antistaphylococcal endopeptidase) for reducing nasal burden of methicillin-resistant Staphylococcus aureus (MRSA). Methods: The community-acquired MRSA strain S. aureus NRS384 (USA300-0114) was used in the present study because it is commonly isolated from human nares and it established consistent and reproducible colonization of cotton rat nares. This model was used to evaluate the efficacy of ranalexin/lysostaphin gels (0.1%-1% w/v; administered intranasally once or once per day for 3 consecutive days) for reducing nasal MRSA burden. Control animals were administered vehicle gel only (0.5% hydroxypropyl methylcellulose) or 2% mupirocin, which is used clinically for nasal decolonization of MRSA. Nasal MRSA burden was assessed at 192 h post-inoculation, which was at least 72 h after the final treatment had been administered. An additional study assessed the efficacy of 0.1% ranalexin/lysostaphin against a mupirocin-resistant MRSA strain (MUP20), which had been selected by serial passage of S. aureus NRS384 through subinhibitory concentrations of mupirocin. Results: Gels containing 0.1% ranalexin/lysostaphin consistently reduced median nasal burden of MRSA to an extent similar to or greater than 2% mupirocin. Treatment with 0.1% ranalexin/lysostaphin was also effective against the MUP20 strain. There was evidence for only minimal irritancy in cotton rat nares administered three doses of 0.1% ranalexin/lysostaphin, suggesting that this agent is suitable for short-course therapy such as is employed currently for nasal decolonization with mupirocin. Conclusions: Ranalexin/lysostaphin could serve as an alternative to mupirocin for nasal decolonization of MRSA.
Type: Journal Article
URI: http://hdl.handle.net/1893/17943
DOI Link: http://dx.doi.org/10.1093/jac/dkt243
Rights: The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.
Affiliation: Aquaculture
Euro-protec Ltd
University of St Andrews
Euro-protec Ltd
University of St Andrews

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