Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/17363
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dc.contributor.authorCarmichael, Stephen Nen_UK
dc.contributor.authorBekaert, Michaëlen_UK
dc.contributor.authorTaggart, Johnen_UK
dc.contributor.authorChristie, Hayden R Len_UK
dc.contributor.authorBassett, D Ien_UK
dc.contributor.authorBron, Jamesen_UK
dc.contributor.authorSkuce, Phillipen_UK
dc.contributor.authorGharbi, Karimen_UK
dc.contributor.authorSkern-Mauritzen, Rasmusen_UK
dc.contributor.authorSturm, Arminen_UK
dc.date.accessioned2017-08-25T01:46:08Z-
dc.date.available2017-08-25T01:46:08Z-
dc.date.issued2013-10en_UK
dc.identifier.othere77832en_UK
dc.identifier.urihttp://hdl.handle.net/1893/17363-
dc.description.abstractThe salmon louse (Lepeophtheirus salmonis (Krøyer, 1837)) is a parasitic copepod that can, if untreated, cause considerable damage to Atlantic salmon (Salmo salar Linnaeus, 1758) and incurs significant costs to the Atlantic salmon mariculture industry. Salmon lice are gonochoristic and normally show sex ratios close to 1:1. While this observation suggests that sex determination in salmon lice is genetic, with only minor environmental influences, the mechanism of sex determination in the salmon louse is unknown. This paper describes the identification of a sex-linked Single Nucleotide Polymorphism (SNP) marker, providing the first evidence for a genetic mechanism of sex determination in the salmon louse. Restriction site-associated DNA sequencing (RAD-seq) was used to isolate SNP markers in a laboratory-maintained salmon louse strain. A total of 85 million raw Illumina 100 base paired-end reads produced 281,838 unique RAD-tags across 24 unrelated individuals. RAD marker Lsa101901 showed complete association with phenotypic sex for all individuals analysed, being heterozygous in females and homozygous in males. Using an allele-specific PCR assay for genotyping, this SNP association pattern was further confirmed for three unrelated salmon louse strains, displaying complete association with phenotypic sex in a total of 96 genotyped individuals. The marker Lsa101901 was located in the coding region of the prohibitin-2 gene, which showed a sex-dependent differential expression, with mRNA levels determined by RT-qPCR about 1.8-fold higher in adult female than adult male salmon lice. This study's observations of a novel sex-linked SNP marker are consistent with sex determination in the salmon louse being genetic and following a female heterozygous system. Marker Lsa101901 provides a tool to determine the genetic sex of salmon lice, and could be useful in the development of control strategies.en_UK
dc.language.isoenen_UK
dc.publisherPublic Library of Scienceen_UK
dc.relationCarmichael SN, Bekaert M, Taggart J, Christie HRL, Bassett DI, Bron J, Skuce P, Gharbi K, Skern-Mauritzen R & Sturm A (2013) Identification of a sex-linked SNP marker in the salmon louse (Lepeophtheirus salmonis) using RAD sequencing. PLoS ONE, 8 (10), Art. No.: e77832. https://doi.org/10.1371/journal.pone.0077832en_UK
dc.rights© 2013 Carmichael et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_UK
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_UK
dc.titleIdentification of a sex-linked SNP marker in the salmon louse (Lepeophtheirus salmonis) using RAD sequencingen_UK
dc.typeJournal Articleen_UK
dc.identifier.doi10.1371/journal.pone.0077832en_UK
dc.citation.jtitlePLoS ONEen_UK
dc.citation.issn1932-6203en_UK
dc.citation.volume8en_UK
dc.citation.issue10en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusVoR - Version of Recorden_UK
dc.author.emaild.i.bassett@stir.ac.uken_UK
dc.contributor.affiliationUniversity of Stirlingen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationUniversity of Stirlingen_UK
dc.contributor.affiliationMachrihanishen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationThe Moredun Research Instituteen_UK
dc.contributor.affiliationUniversity of Edinburghen_UK
dc.contributor.affiliationNorwegian Institute of Marine Researchen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.identifier.isiWOS:000326019400138en_UK
dc.identifier.scopusid2-s2.0-84885703379en_UK
dc.identifier.wtid674551en_UK
dc.contributor.orcid0000-0002-1206-7654en_UK
dc.contributor.orcid0000-0002-3843-9663en_UK
dc.contributor.orcid0000-0002-7529-0829en_UK
dc.contributor.orcid0000-0003-3544-0519en_UK
dc.contributor.orcid0000-0003-2632-1999en_UK
dc.date.accepted2013-09-13en_UK
dcterms.dateAccepted2013-09-13en_UK
dc.date.filedepositdate2013-11-01en_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionVoRen_UK
local.rioxx.authorCarmichael, Stephen N|en_UK
local.rioxx.authorBekaert, Michaël|0000-0002-1206-7654en_UK
local.rioxx.authorTaggart, John|0000-0002-3843-9663en_UK
local.rioxx.authorChristie, Hayden R L|en_UK
local.rioxx.authorBassett, D I|0000-0002-7529-0829en_UK
local.rioxx.authorBron, James|0000-0003-3544-0519en_UK
local.rioxx.authorSkuce, Phillip|en_UK
local.rioxx.authorGharbi, Karim|en_UK
local.rioxx.authorSkern-Mauritzen, Rasmus|en_UK
local.rioxx.authorSturm, Armin|0000-0003-2632-1999en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate2013-11-01en_UK
local.rioxx.licencehttp://creativecommons.org/licenses/by/4.0/|2013-11-01|en_UK
local.rioxx.filenameCarmichael 2013 sex linked SNP.pdfen_UK
local.rioxx.filecount1en_UK
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