Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/1705
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dc.contributor.authorBury, Nicholas Ren_UK
dc.contributor.authorChung, Mi Jaen_UK
dc.contributor.authorSturm, Arminen_UK
dc.contributor.authorWalker, Paul Aen_UK
dc.contributor.authorHogstrand, Christeren_UK
dc.date.accessioned2012-09-03T12:49:41Z-
dc.date.available2012-09-03T12:49:41Zen_UK
dc.date.issued2008-02en_UK
dc.identifier.urihttp://hdl.handle.net/1893/1705-
dc.description.abstractIntracellular zinc signaling is important in the control of a number of cellular processes. Hormonal factors that regulate cellular zinc influx and initiate zinc signals are poorly understood. The present study investigates the possibility for cross talk between the glucocorticoid and zinc signaling pathways in cultured rainbow trout gill epithelial cells. The rainbow trout metallothionein A (MTA) gene possesses a putative glucocorticoid response element and multiple metal response elements 1042 base pairs upstream of the start codon, whereas metallothionein B (MTB) and zinc transporter-1 (ZnT1) have multiple metal response elements but no glucocorticoid response elements in this region. Cortisol increased MTA, MTB, and ZnT1 gene expression, and this stimulation was enhanced if cells were treated with cortisol together with zinc. Cells treated with zinc showed increased zinc accumulation, transepithelial zinc influx (apical to basolateral), and intracellular labile zinc concentrations. These responses were also significantly enhanced in cells pretreated with cortisol and zinc. The cortisol-mediated effects were blocked by the glucocorticoid receptor (GR) antagonist RU-486, indicating mediation via a GR. In reporter gene assays, zinc stimulated MTA promoter activity, whereas cortisol did not. Furthermore, cortisol significantly reduced zinc-stimulated MTA promoter activity in cells expressing exogenous rainbow trout GR. These results demonstrate that cortisol enhances cellular zinc uptake, which in turn stimulates expression of MTA, MTB, and ZnT1 genes.en_UK
dc.language.isoenen_UK
dc.publisherAmerican Physiological Societyen_UK
dc.relationBury NR, Chung MJ, Sturm A, Walker PA & Hogstrand C (2008) Cortisol stimulates the zinc signaling pathway and expression of metallothioneins and ZnT1 in rainbow trout gill epithelial cells. American Journal of Physiology - Regulatory, Integrative and Comparative Physiology, 294 (2), pp. R623-R629. http://ajpregu.physiology.org/; https://doi.org/10.1152/ajpregu.00646.2007en_UK
dc.rightsCopyright by American Physiological Society; The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author; you can only request a copy if you wish to use this work for your own research or private study.en_UK
dc.rights.urihttp://www.rioxx.net/licenses/under-embargo-all-rights-reserveden_UK
dc.subjectfishen_UK
dc.subjectmetalsen_UK
dc.subjectglucocorticoid receptoren_UK
dc.subjectRainbow trout Anatomyen_UK
dc.subjectGlucocorticoids Receptorsen_UK
dc.titleCortisol stimulates the zinc signaling pathway and expression of metallothioneins and ZnT1 in rainbow trout gill epithelial cellsen_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate2999-12-13en_UK
dc.rights.embargoreason[Bury2007.pdf] The publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work.en_UK
dc.identifier.doi10.1152/ajpregu.00646.2007en_UK
dc.citation.jtitleAmerican journal of physiology: regulatory, integrative and comparative physiologyen_UK
dc.citation.issn1522-1490en_UK
dc.citation.issn0363-6119en_UK
dc.citation.volume294en_UK
dc.citation.issue2en_UK
dc.citation.spageR623en_UK
dc.citation.epageR629en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusVoR - Version of Recorden_UK
dc.identifier.urlhttp://ajpregu.physiology.org/en_UK
dc.author.emailas49@stir.ac.uken_UK
dc.citation.date12/12/2007en_UK
dc.contributor.affiliationKing's College Londonen_UK
dc.contributor.affiliationKing's College Londonen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationKing's College Londonen_UK
dc.contributor.affiliationKing's College Londonen_UK
dc.identifier.wtid835714en_UK
dc.contributor.orcid0000-0003-2632-1999en_UK
dcterms.dateAccepted2007-12-12en_UK
dc.date.filedepositdate2009-10-15en_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionVoRen_UK
local.rioxx.authorBury, Nicholas R|en_UK
local.rioxx.authorChung, Mi Ja|en_UK
local.rioxx.authorSturm, Armin|0000-0003-2632-1999en_UK
local.rioxx.authorWalker, Paul A|en_UK
local.rioxx.authorHogstrand, Christer|en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate2999-12-13en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/under-embargo-all-rights-reserved||en_UK
local.rioxx.filenameBury2007.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source0363-6119en_UK
Appears in Collections:Aquaculture Journal Articles

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