Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/1703
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Plasma 11-deoxycorticosterone (DOC) and mineralocorticoid receptor testicular expression during rainbow trout Oncorhynchus mykiss spermiation: implication with 17alpha, 20beta-dihydroxyprogesterone on the milt fluidity?
Authors: Milla, Sylvain
Terrien, Xavier
Sturm, Armin
Ibrahim, Fidaa
Giton, Franck
Fiet, Jean
Prunet, Patrick
Le, Gac Florence
Contact Email: as49@stir.ac.uk
Keywords: fish
endocronology
testis
sperm
maturation
reproduction
Issue Date: May-2008
Publisher: BioMed Central
Citation: Milla S, Terrien X, Sturm A, Ibrahim F, Giton F, Fiet J, Prunet P & Le Gac F (2008) Plasma 11-deoxycorticosterone (DOC) and mineralocorticoid receptor testicular expression during rainbow trout Oncorhynchus mykiss spermiation: implication with 17alpha, 20beta-dihydroxyprogesterone on the milt fluidity?, Reproductive Biology and Endocrinology, 6 (1).
Abstract: Background: In rainbow trout (Oncorhynchus mykiss), the endocrine control of spermiation is not fully understood. Besides IIketotestosterone (IIKT) and 17alpha, 20beta-dihydroxyprogesterone (MIS), the potential physiological ligand of the mineralocorticoid receptor (MR) II-deoxycorticosterone (DOC), is a credible candidate in O. mykiss spermiation regulation as spermiation is accompanied with changes in aqueous and ionic flows. Methods: In this study, we investigated potential roles of DOC during spermiation 1) by describing changes in blood plasma DOC level, MR mRNA abundance during the reproductive cycle and MR localization in the reproductive tract 2) by investigating and comparing the effects of DOC (10 mg/kg) and MIS (5 mg/kg) supplementations on sperm parameters 3) by measuring the in vitro effect of DOC on testis MIS production. Results: The plasma concentration of DOC increased rapidly at the end of the reproductive cycle to reach levels that were 10-50 fold higher in mature males than in immature fish. MR mRNA relative abundance was lower in maturing testes when compared to immature testes, but increased rapidly during the spermiation period, immediately after the plasma rise in DOC. At this stage, immunohistochemistry localized MR protein to cells situated at the periphery of the seminiferous tubules and in the efferent ducts. Neither DOC nor MIS had significant effects on the mean sperm volume, although MIS treatment significantly increased the percentage of males producing milt. However, a significant reduction in the spermatocrit was observed when DOC and MIS were administrated together. Finally, we detected an inhibitory effect of DOC on testis MIS production in vitro. Conclusion: These results are in agreement with potential roles of DOC and MR during spermiation and support the hypothesis that DOC and MIS mechanisms of action are linked during this reproductive stage, maybe controlling milt fluidity. They also confirm that in O. mykiss MIS is involved in spermiation induction.
Type: Journal Article
URI: http://hdl.handle.net/1893/1703
URL: http://www.rbej.com/content/6/1/19
DOI Link: http://dx.doi.org/10.1186/1477-7827-6-19
Rights: copyright by BioMed Central; Published in Reproductive Biology and Endocrinology by BioMed Central.; © 2008 Milla et al; licensee BioMed Central Ltd. http://www.biomedcentral.com/info/about/license; Open Access. Publisher statement: "This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited".
Affiliation: Institut National de la Recherche Agronomique
Institut National de la Recherche Agronomique
Aquaculture
Hôpital Saint-Louis
Hopital Henri Mondor, Faculte de Medecine
Hopital Henri Mondor, Faculte de Medecine
Institut National de la Recherche Agronomique
Institut National de la Recherche Agronomique

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