Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/16004
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Mapping and Validation of the Major Sex-Determining Region in Nile Tilapia (Oreochromis niloticus L.) Using RAD Sequencing
Authors: Palaiokostas, Christos
Bekaert, Michaël
Khan, Mohd Golam Quader
Taggart, John
Gharbi, Karim
McAndrew, Brendan
Penman, David
Contact Email: d.j.penman@stir.ac.uk
Keywords: Oreochromis niloticus
Sex Determination
QTL Mapping
RAD-seq
Aquaculture
Issue Date: Jul-2013
Publisher: Public Library of Science
Citation: Palaiokostas C, Bekaert M, Khan MGQ, Taggart J, Gharbi K, McAndrew B & Penman D (2013) Mapping and Validation of the Major Sex-Determining Region in Nile Tilapia (Oreochromis niloticus L.) Using RAD Sequencing, PLoS ONE, 8 (7), Art. No.: e68389.
Abstract: Sex in Oreochromis niloticus (Nile tilapia) is principally determined by an XX/XY locus but other genetic and environmental factors also influence sex ratio. Restriction Associated DNA (RAD) sequencing was used in two families derived from crossing XY males with females from an isogenic clonal line, in order to identify Single Nucleotide Polymorphisms (SNPs) and map the sex-determining region(s). We constructed a linkage map with 3,802 SNPs, which corresponded to 3,280 informative markers, and identified a major sex-determining region on linkage group 1, explaining nearly 96% of the phenotypic variance. This sex-determining region was mapped in a 2 cM interval, corresponding to approximately 1.2 Mb in the O. niloticus draft genome. In order to validate this, a diverse family (4 families; 96 individuals in total) and population (40 broodstock individuals) test panel were genotyped for five of the SNPs showing the highest association with phenotypic sex. From the expanded data set, SNPs Oni23063 and Oni28137 showed the highest association, which persisted both in the case of family and population data. Across the entire dataset all females were found to be homozygous for these two SNPs. Males were heterozygous, with the exception of five individuals in the population and two in the family dataset. These fish possessed the homozygous genotype expected of females. Progeny sex ratios (over 95% females) from two of the males with the "female" genotype indicated that they were neomales (XX males). Sex reversal induced by elevated temperature during sexual differentiation also resulted in phenotypic males with the "female" genotype. This study narrows down the region containing the main sex-determining locus, and provides genetic markers tightly linked to this locus, with an association that persisted across the population. These markers will be of use in refining the production of genetically male O. niloticus for aquaculture.
Type: Journal Article
URI: http://hdl.handle.net/1893/16004
URL: http://dx.doi.org/10.1371/journal.pone.0068389
DOI Link: http://dx.doi.org/10.1371/journal.pone.0068389
Rights: Copyright: © 2013 Palaiokostas et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Affiliation: Aquaculture
Aquaculture
Bangladesh Agricultural University
Aquaculture
University of Edinburgh
Aquaculture
Aquaculture

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