Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/15457
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Production and efficacy of an Aeromonas hydrophila recombinant S-layer protein vaccine for fish
Authors: Poobalane, Saravanane
Thompson, Kimberly
Ardo, Laszlo
Verjan, Noel
Han, Hyun-Ja
Jeney, Galina
Hirono, Ikuo
Aoki, Takashi
Adams, Alexandra
Contact Email: alexandra.adams@stir.ac.uk
Keywords: Aeromonas hydrophila
Recombinant S-layer protein vaccine
Efficacy testing
Common carp
Issue Date: Apr-2010
Publisher: Elsevier
Citation: Poobalane S, Thompson K, Ardo L, Verjan N, Han H, Jeney G, Hirono I, Aoki T & Adams A (2010) Production and efficacy of an Aeromonas hydrophila recombinant S-layer protein vaccine for fish, Vaccine, 28 (20), pp. 3540-3547.
Abstract: A recombinant protein for the S-layer protein of Aeromonas hydrophila was produced and its ability to protect common carp Cyprinus carpio L. against six virulent isolates of A. hydrophila was assessed. A group of 120 carp (30-40 g) were vaccinated intra-peritoneally with 0.1 ml of adjuvanted vaccine (30 μg protein per fish). Another group of 120 carp were injected with 0.1 ml of PBS-adjuvant mixture to serve as controls. Twenty fish from each group were challenged with each one of six virulent isolates of A. hydrophila 35 days post-vaccination. The fish were maintained in 12 separate tanks before terminating the experiment at 16 days post-challenge. The relative percentage survival (RPS) for the six isolates of A. hydrophila ranged from 56 to 87%. The difference in survival rate of fish challenged with four of the isolates was statistically significant in vaccinated fish compared to control fish, when analysed using a Chi-square test. The results of the study suggest that the recombinant S-layer protein of A. hydrophila could be useful as a vaccine antigen to protect fish against different isolates of this pathogenic bacterium.
Type: Journal Article
URI: http://hdl.handle.net/1893/15457
DOI Link: http://dx.doi.org/10.1016/j.vaccine.2010.03.011
Rights: The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.
Affiliation: Aquaculture
Aquaculture
Research Institute Fisheries, Aquaculture and Irrigation
Tokyo University of Marine Science and Technology
Tokyo University of Marine Science and Technology
Research Institute Fisheries, Aquaculture and Irrigation
Tokyo University of Marine Science and Technology
Tokyo University of Fisheries
Aquaculture

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