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Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Ontogenic effects of early feeding of sea bass (Dicentrarchus labrax) larvae with a range of dietary n-3 HUFA levels on the functioning of PUFA desaturation pathways
Authors: Vagner, Marie
Robin, Jean H
Zambonino-Infante, Jose L
Tocher, Douglas R
Person-Le, Ruyet Jeannine
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Keywords: sea bass
delta-6 desaturase
Issue Date: May-2009
Citation: Vagner M, Robin JH, Zambonino-Infante JL, Tocher DR & Person-Le Ruyet J (2009) Ontogenic effects of early feeding of sea bass (Dicentrarchus labrax) larvae with a range of dietary n-3 HUFA levels on the functioning of PUFA desaturation pathways, British Journal of Nutrition, 101 (10), pp. 1452-1462.
Abstract: Four replicated groups of sea bass (Dicentrarchus labrax) larvae were fed diets containing an extra-high level of highly unsaturated fatty acids (HUFA) (XH; 3·7% EPA + DHA), a high level of HUFA (HH; 1·7 %), a low level of HUFA (LH; 0·7%) or an extra-low level of HUFA (XLH; 0·5%) from day 6 to day 45 (experiment 1; XH1, HH1, LH1, XLH1). After a subsequent 1-month period feeding a commercial diet (2·7% EPA + DHA), the capacity of the four initial groups to adapt to an n-3 HUFA-restricted diet (0·3% EPA + DHA; R-groups: XH2R, HH2R, LH2R, XLH2R) was tested for 35 d. Larval dietary treatments had no effect on larval and juvenile survival rates. The wet weight of day 45 larvae was higher in XH1 and HH1 (P < 0·001), but the R-juvenile mass gains were similar in all treatments. D-6-desaturase (D6D) mRNA level was higher in LH1 and XLH1 at day 45 (P < 0·001), and higher in LH2R and XLH2R, with a significant increase at day 118.Concomitantly, PPARa and PPARb mRNA levels were higher in XLH1 at day 45, and PPARb and g mRNA levels were higher in XLH2R at day 118, suggesting possible involvement of PPAR in stimulation of D6D expression, when drastic dietary larval conditioning occurred. The low DHA content in the polar lipids (PL) of LH1 and XLH1 revealed an n-3-HUFA deficiency in these groups. Larval conditioning did not affect DHA content in the PL of R-juveniles. The present study showed (i) a persistent D6D mRNA enhancement in juveniles pre-conditioned with an n-3 HUFA-deficient larval diet, over the 1-month intermediate period, and (ii) brought new findings suggesting the involvement of PPAR in the D6D mRNA level stimulation. However, such nutritional conditioning had no significant effect on juvenile growth and lipid composition.
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