Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/13013
Appears in Collections:Faculty of Health Sciences and Sport Journal Articles
Peer Review Status: Refereed
Title: Comparison of an enzyme immunoassay for the detection of Helicobacter pylori antigens in the faeces with the urea breath test
Authors: Shepherd, Ashley
Williams, Craig L
Doherty, Conor P
Hossack, Margaret
Preston, Tom
Mccoll, Kenneth E L
Weaver, Lawrence T
Contact Email: ashley.shepherd@stir.ac.uk
Keywords: Helicobacter pylori
faeces
ELISA
urea breath test
Issue Date: Sep-2000
Publisher: BMJ Publishing Group for the Royal College of Paediatrics and Child Health and the European Academy of Paediatrics
Citation: Shepherd A, Williams CL, Doherty CP, Hossack M, Preston T, Mccoll KEL & Weaver LT (2000) Comparison of an enzyme immunoassay for the detection of Helicobacter pylori antigens in the faeces with the urea breath test, Archives of Disease in Childhood, 83 (3), pp. 268-270.
Abstract: BACKGROUND: Current diagnostic tests for Helicobacter pylori are invasive (endoscopy) or indirect (urea breath test, serology). AIMS: To evaluate a new enzyme immunoassay (EIA) which detects H pyloriantigens in faeces, by comparing its sensitivity and specificity in children with the 13C urea breath test (UBT). METHODS: A total of 119 children underwent a UBT and provided a faecal sample for antigen testing within seven days. After an overnight fast each child provided a pretest breath sample, and samples at 30 and 40 minutes after ingestion of 100 mg 13C labelled urea. 13C enrichment of breath was measured by isotope ratio mass spectrometry. Faeces were stored at -70°C until antigen testing, using the EIA. Samples were read spectrophotometrically at 450 nm and results were interpreted using recommended cut offs of optical density less than 0.14 as negative, ⩾0.16 as positive, with ⩾0.14 and less than 0.16 representing equivocal results. Sensitivity and specificity were calculated using the manufacturer's cut off compared with UBT. RESULTS: Sensitivity and specificity were 88% and 82%, respectively. Negative and positive predictive values were 97% and 58%. CONCLUSIONS: The EIA is an alternative, non-invasive, and easy to use method for the detection ofH pylori in children. Its high negative predictive value suggests a role in screening out uninfected children.
Type: Journal Article
URI: http://hdl.handle.net/1893/13013
DOI Link: http://dx.doi.org/10.1136/adc.83.3.268
Rights: The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.
Affiliation: HS Research - Stirling
University of Glasgow
University of Glasgow
University of Glasgow
Scottish Universities Environmental Research Centre
University of Glasgow
University of Glasgow

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