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Appears in Collections:Faculty of Health Sciences and Sport Journal Articles
Peer Review Status: Refereed
Title: Myogenic gene expression signature establishes that brown and white adipocytes originate from distinct cell lineages
Authors: Timmons, James A
Wennmalm, Kristian
Larsson, Ola
Walden, Tomas B
Lassmann, Timo
Petrovic, Natasa
Hamilton, David Lee
Gimeno, Ruth E
Wahlestedt, Claes
Baar, Keith
Nedergaard, Jan
Cannon, Barbara
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Keywords: microarray
principal component analysis
Issue Date: 13-Mar-2007
Publisher: National Academy of Sciences
Citation: Timmons JA, Wennmalm K, Larsson O, Walden TB, Lassmann T, Petrovic N, Hamilton DL, Gimeno RE, Wahlestedt C, Baar K, Nedergaard J & Cannon B (2007) Myogenic gene expression signature establishes that brown and white adipocytes originate from distinct cell lineages, Proceedings of the National Academy of Sciences, 104 (11), pp. 4401-4406.
Abstract: Attainment of a brown adipocyte cell phenotype in white adipocytes, with their abundant mitochondria and increased energy expenditure potential, is a legitimate strategy for combating obesity. The unique transcriptional regulators of the primary brown adipocyte phenotype are unknown, limiting our ability to promote brown adipogenesis over white. In the present work, we used microarray analysis strategies to study primary preadipocytes, and we made the striking discovery that brown preadipocytes demonstrate a myogenic transcriptional signature, whereas both brown and white primary preadipocytes demonstrate signatures distinct from those found in immortalized adipogenic models. We found a plausible SIRT1-related transcriptional signature during brown adipocyte differentiation that may contribute to silencing the myogenic signature. In contrast to brown preadipocytes or skeletal muscle cells, white preadipocytes express Tcf21, a transcription factor that has been shown to suppress myogenesis and nuclear receptor activity. In addition, we identified a number of developmental genes that are differentially expressed between brown and white preadipocytes and that have recently been implicated in human obesity. The interlinkage between the myocyte and the brown preadipocyte confirms the distinct origin for brown versus white adipose tissue and also represents a plausible explanation as to why brown adipocytes ultimately specialize in lipid catabolism rather than storage, much like oxidative skeletal muscle tissue.
Type: Journal Article
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Rights: The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.
Notes: Muscle strength; Isometric exercise
Affiliation: Stockholm University
Karolinska Institutet
Karolinska Institutet
Stockholm University
Karolinska Institutet
Stockholm University
Millennium Pharmaceuticals, Cambridge
Karolinska Institutet
University of Dundee
Stockholm University
Stockholm University

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