Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/11485
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Kisspeptin and seasonal control of reproduction in male European sea bass (Dicentrarchus labrax)
Authors: Migaud, Herve
Ismail, Rania F K
Cowan, Mairi
Davie, Andrew
Contact Email: andrew.davie@stir.ac.uk
Keywords: Kisspeptin
GnRH
Brain
Sea bass
Dicentrarchus labrax
Reproduction
Issue Date: Dec-2012
Publisher: Elsevier
Citation: Migaud H, Ismail RFK, Cowan M & Davie A (2012) Kisspeptin and seasonal control of reproduction in male European sea bass (Dicentrarchus labrax), General and Comparative Endocrinology, 179 (3), pp. 384-399.
Abstract: In the present study, we developed and validated real-time quantitative RT-PCR assays for a suite of genes involved in the brain-pituitary gonadal axis in fish including kisspeptin genes and its receptor (Kiss1, kiss2, kissr4) and gonadotropin-releasing hormone genes (sbGnRH, sGnRH, cGnRHII) in the brain, and gonadotropin genes (fshβ and lhβ) in the pituitary. Sex steroid profiles (T and 11-KT) and gonadal development were also studied over a full annual reproductive cycle in adult male sea bass. The cDNA partial sequence of sea bass kissr4 encoding 185 amino acids showed a high degree of conservation with other fish kissr4 subtype. Results clearly showed a seasonal profile for Kiss1, kiss2 and kissr4 mRNAs. Kissr4, fshβ and lhβ levels increased gradually and peaked during spermatogenesis (January) while Kiss1, kiss2, cGnRH-II as well as steroids showed peaks during early spawning (March). No significant seasonal changes were observed for sbGnRH and sGnRH expression. These results support the possible involvement of the kiss genes and their receptor (kissr4) in the seasonal control sea bass reproduction. However, a lack of correlation between kiss genes and sbGnRH expression and the mismatch between kisspeptin and the onset of gonadotropin surge contrast with previous findings.
Type: Journal Article
URI: http://hdl.handle.net/1893/11485
DOI Link: http://dx.doi.org/10.1016/j.ygcen.2012.07.033
Rights: The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.
Affiliation: Aquaculture
University of Stirling
Aquaculture (Machrihanish)
Aquaculture

Files in This Item:
File Description SizeFormat 
Kisspeptin and seasonal control.pdf1.86 MBAdobe PDFUnder Embargo until 31/12/2999     Request a copy

Note: If any of the files in this item are currently embargoed, you can request a copy directly from the author by clicking the padlock icon above. However, this facility is dependant on the depositor still being contactable at their original email address.

This item is protected by original copyright



Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

If you believe that any material held in STORRE infringes copyright, please contact library@stir.ac.uk providing details and we will remove the Work from public display in STORRE and investigate your claim.